Paxillin [p Tyr118] Antibody (2E6P0)

Western Blot: Paxillin [p Tyr118] Antibody (2E6P0) [NBP3-16929] - Western blot analysis of extracts of various cell lines, using Paxillin Rabbit mAb (NBP3-16929) at 1:1000 dilution.A-431 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight.C6 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 2E6P0
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Paxillin [p Tyr118] Antibody (2E6P0) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic phosphorylated peptide around Y118 of human Paxillin (P49023). HVYSF
• Modification: p Tyr118
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PXN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Paxillin [p Tyr118] Antibody (2E6P0)

• FLJ16691
• Paxillin
• PXN

Background

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PXN