Patched 2/PTCH2 Antibody (865619) [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: IHC
• Clone: 865619
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

Patched 2/PTCH2 Antibody (865619) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant mouse Patched 2/PTCH2
  Glu79-Ser394
  Accession # O35595
• Specificity: Detects mouse Patched 2/PTCH2 in ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Patched 1 or recombinant human Patched 2 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Patched 2/PTCH2 Antibody (865619) [Alexa Fluor® 647]

• patched (Drosophila) homolog 2
• Patched 2
• patched homolog 2 (Drosophila)
• patched homolog 2
• protein patched homolog 2
• ptc2
• PTCH2

Background

Patched 2 (Ptch2) is a 180-200 kDa 12 transmembrane member of the Patched family. It is less widely expressed than Patched 1, but is found at high levels in skin and spermatocytes. The 1182 amino acid (aa) mouse Patched 2 contains two terminal cytoplasmic domains between aa 1-57 and aa 1108-1182. Together with Smoothened (SMO), Patched 2 forms a membrane complex that serves as a receptor for Sonic Hedgehog (Shh). Over the first extracellular loop (aa 79-394), mouse Patched 2 shares 95% aa sequence identity with human Patched 2. Mouse Patched 2 has one potential 1117 aa isoform that lacks aa 207-271.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.