Patched 1/PTCH Antibody

Western Blot: Patched 1/PTCH Antibody [NB100-41101] - Staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody [NB100-41101] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Po, Bv, Ca
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Patched 1/PTCH Antibody Summary

• Immunogen: Peptide with sequence C-HPESRHHPPSNPRQQ corresponding to internal region according to NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1.
• Specificity: This antibody is expected to recognise all four reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1). Reported variants represent identical protein (NP_001077075.1; NP_001077076.1; NP_001077073.1; NP_001077074.1).
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTCH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx 150 kDa band observed in human brain lysates (calculated MW of 153.9 kDa according to NP_001077071.1).
• Control:

  Patched 1/PTCH Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Patched 1/PTCH Antibody

• BCNS
• BCNSFLJ42602
• FLJ26746
• HPE7
• mes
• NBCCS
• patched (Drosophila) homolog
• Patched 1
• patched homolog (Drosophila)
• patched homolog 1 (Drosophila)
• patched homolog 1
• protein patched homolog 1
• PTC
• ptc1
• PTCH protein +12b
• PTCH protein +4'
• PTCH protein -10
• PTCH
• PTCH1
• PTCH11

Background

This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis. This gene functions as a tumor suppressor. Mutations of this gene have been associated with nevoid basal cell carcinoma syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Patched 1/PTCH Antibody (NB100-41101)(1)

Publication using NB100-41101

• Nagao K, Togawa N, Fujii K et al. Detecting tissue-specific alternative splicing and disease-associated aberrant splicing of the PTCH gene with exon junction microarrays. Hum Mol Genet 2005-10-03 [PMID: 16203740]

FAQs for Patched 1/PTCH Antibody (NB100-41101). (Showing 1 - 1 of 1 FAQ).

1. I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
   • NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.

Bioinformatics

• Gene Symbol: PTCH1
• Entrez:
  • 5727(Human)
• Uniprot:
  • NP_000255.1(Human)