Patched 1/PTCH Antibody

Images

 
Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451] - (3.75ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451] - (3.8ug/ml) Staining of Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. In paraffin embedded Human Colon shows textured ...read more
Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Patched 1/PTCH Antibody Summary

Immunogen
Peptide with sequence ELQDVECEERPR corresponding to C-Terminus according to NP_000255.1, NP_001077072.1, NP_001077071.1, NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1.
Specificity
This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PTCH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
IHC-P: Human colon shows textured nuclear staining of the absorptive cells. IHC successful usage on human glioma sections is reported in scientific literature (PMID: 24867209).
Positive Control
Patched 1/PTCH Lysate (NBL1-14924)
Publications
Read Publication using NB100-2451.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Patched 1/PTCH Antibody

  • BCNS
  • BCNSFLJ42602
  • FLJ26746
  • HPE7
  • mes
  • NBCCS
  • patched (Drosophila) homolog
  • Patched 1
  • patched homolog (Drosophila)
  • patched homolog 1 (Drosophila)
  • patched homolog 1
  • protein patched homolog 1
  • PTC
  • ptc1
  • PTCH protein +12b
  • PTCH protein +4'
  • PTCH protein -10
  • PTCH
  • PTCH1
  • PTCH11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Patched 1/PTCH Antibody (NB100-2451)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Patched 1/PTCH Antibody (NB100-2451). (Showing 1 - 1 of 1 FAQ).

  1. I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
    • NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PTCH1
Entrez
Uniprot