Patched 1/PTCH Antibody (413220) [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 413220
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 700

Patched 1/PTCH Antibody (413220) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant mouse Patched 1/PTCH
  Glu108-Asp422
  Accession # Q61115
• Specificity: Detects mouse Patched 1/PTCH (First Extracellular Loop) in direct ELISAs and Western blots. In direct ELISAs and Western blots, 15% cross‑reactivity with recombinant human Patched 2 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Patched 1/PTCH Antibody (413220) [Alexa Fluor® 700]

• BCNS
• BCNSFLJ42602
• FLJ26746
• HPE7
• mes
• NBCCS
• patched (Drosophila) homolog
• Patched 1
• patched homolog (Drosophila)
• patched homolog 1 (Drosophila)
• patched homolog 1
• protein patched homolog 1
• PTC
• ptc1
• PTCH protein +12b
• PTCH protein +4'
• PTCH protein -10
• PTCH
• PTCH1
• PTCH11

Background

Patched homolog 1 (PTCH, also called PTC, PTC1 or PTCH1) is a 170‑175 kDa member of the Patched family of proteins. It is found on multiple cells types and forms a constitutive, inactivating complex with Smoothened. Upon SHH binding to PTC1, Smoothened is released and initiates downstream signaling. Mouse PTCH is a 12 transmembrane, 1434 aa glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for PTCH has been reported in mice. However, it would appear likely that two or more splice forms exist, with variation occurring over the first 150 aa of the N-terminus. Over aa 108‑422, mouse PTCH shares 97% and 99% aa sequence identity with human and rat PTCH, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Patched 1/PTCH Antibody (FAB41051N). (Showing 1 - 1 of 1 FAQ).

1. I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
   • NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.