PASD1 Antibody (2ALCC136) - BSA Free

Western Blot: PASD1 Antibody (2ALCC136) [NBP2-50235] - Western Blotting PASD1 silencing with three independent PASD1 siRNAs was performed to validate the identity of the endogenous protein detected by Western Blotting in BT-20 breast cancer cells. Controls from left to right are untreated, mock siRNA, control siRNA. Experiments in other cell types have given similar results. There may be some variability in the m.wt. of PASD1, 100-150kDa.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: 2ALCC136
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PASD1 Antibody (2ALCC136) - BSA Free Summary

• Description: Novus Biologicals Mouse PASD1 Antibody (2ALCC136) - BSA Free (NBP2-50235) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: GST-PASD1 recombinant protein containing 195-474 aas of PASD1. Region is common to PASD1a and PASD1b proteins.
• Specificity: PASD1-1 recognizes the recombinant protein (aa 195-474) present in both PASD1a and PASD1b proteins.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: PASD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive control(s): PASD-1 transfected cells and testis; BT-20 and NCI-H1299 carcinoma lines (negative control: MDA-MB-231, tonsil). Antibody PASD1-1 stains nuclei of a subpopulation of spermatogonia near the basal membrane in testicular tubules, with decreasing intensity of labeling with maturity. IHC stainings may be performed on FFPE and cryostat preparations of cells and tissues. Subclass is IgG2a/IgM mixture despite repeated re-cloning. In WB, expected MW is 87.5 kDa/72.5 kDa, observed MW is ~100 kDa.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PASD1 Antibody (2ALCC136) - BSA Free

• CT63Cancer/testis antigen 63
• OXTES1
• OX-TES-1
• PAS domain containing 1
• PAS domain-containing protein 1

Background

PASD1 may function as a transcription factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PASD1