PARP3 Antibody - BSA Free

Western Blot: PARP3 Antibody [NBP2-93033] - Western blot analysis of extracts of Rat liver, using PARP3 antibody (NBP2-93033) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry/Immunofluorescence: PARP3 Antibody [NBP2-93033] - Analysis of MCF-7 cells using PARP3 . Blue: DAPI for nuclear staining.

Immunohistochemistry: PARP3 Antibody - BSA Free [NBP2-93033] - Immunohistochemistry analysis of PARP3 in paraffin-embedded Rat colon tissue using PARP3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP3 Antibody - BSA Free [NBP2-93033] - Immunohistochemistry analysis of PARP3 in paraffin-embedded Mouse kidney tissue using PARP3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: PARP3 Antibody - BSA Free [NBP2-93033] - Immunohistochemistry analysis of PARP3 in paraffin-embedded Human colon tissue using PARP3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PARP3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PARP3 Antibody - BSA Free (NBP2-93033) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 301-540 of human PARP3 (NP_001003931.3). LAQALQAVSEQEKTVEEVPHPLDRDYQLLKCQLQLLDSGAPEYKVIQTYLEQTGSNHRCPTLQHIWKVNQEGEEDRFQAHSKLGNRKLLWHGTNMAVVAAILTSGLRIMPHSGGRVGKGIYFASENSKSAGYVIGMKCGAHHVGYMFLGEVALGREHHINTDNPSLKSPPPGFDSVIARGHTEPDPTQDTELELDGQQVVVPQGQPVPCPEFSSSTFSQSEYLIYQESQCRLRYLLEVHL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PARP3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PARP3 Antibody - BSA Free

• ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 3
• ADPRT3
• ADPRT-3
• ADPRT3PADPRT-3
• ADPRTL2
• ADPRTL3
• ADPRTL3NAD+ ADP-ribosyltransferase 3
• ARTD3
• EC 2.4.2.30
• hPARP-3
• IRT1ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 2
• NAD(+) ADP-ribosyltransferase 3
• pADPRT-3
• PARP3
• PARP-3
• poly (ADP-ribose) polymerase family, member 3
• poly [ADP-ribose] polymerase 3
• poly(ADP-ribose) synthetase-3
• Poly[ADP-ribose] synthase 3
• poly[ADP-ribose] synthetase 3

Background

PARP3 is encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PARP3