PARC Antibody (3F7) - Azide and BSA Free

Western Blot: PARC Antibody (3F7) [H00023113-M01] - C monoclonal antibody (M01), clone 3F7 Western Blot analysis of PARC expression in A-431 ( Cat # L015V1 ).

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PARC Antibody (3F7) - Azide and BSA Free Summary

• Immunogen: PARC (NP_055904, 918 a.a. ~ 1025 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMLLNRYSEPPGSPERAALETPIIQGQDGSPELLIRSLVGGPSAELLLDLERVLCREGSPGGAVRPLLKRLQQETQPFLLLLRTLDAPGPNKTLLLSVLRVITRLLDF
• Specificity: PARC (3F7)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CUL9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
• Control:

  A-431 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PARC Antibody (3F7) - Azide and BSA Free

• CUL-9
• cullin 9
• cullin-9
• DKFZp686G1042
• H7AP1DKFZp686P2024
• p53-associated parkin-like cytoplasmic protein
• PARCKIAA0708
• parkin-like cytoplasmic p53 binding protein
• RP3-330M21.2
• UbcH7-associated protein 1

Background

PARC is a cytoplasmic anchor protein in p53 associated protein complexes. PARC directly interacted and formed an approximately 1MD complex with p53 in the cytoplasm of unstressed cells. In the absence of stress, inactivation of PARC induced nuclear localization of endogenous p53 and activated p53 dependent apoptosis. Overexpression of PARC promoted cytoplasmic sequestration of ectopic p53. Furthermore, abnormal cytoplasmic localization of p53 was observed in a number of neuroblastoma cell lines; RNA interference-mediated reduction of endogenous PARC significantly sensitized these neuroblastoma cells in the DNA damage response. These results revealed that PARC is a critical regulator in controlling p53 subcellular localization and subsequent function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CUL9
• Entrez:
  • 23113(Human)
• OMIM:
  • 607489(Human)
• Uniprot:
  • NP_055904(Human)