PAR1/Thrombin Receptor Antibody

Western Blot: PAR1/Thrombin Receptor Antibody [NBP1-51936] - Staining of HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. ~48 kDa band observed (calculated MW of 47.4 kDa band according to NP_001983.2).

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PAR1/Thrombin Receptor Antibody Summary

• Immunogen: Peptide with sequence C-TRARRPESKATNAT corresponding to internal region (near N-Terminus) according to NP_001983.2.
• Epitope: C-TRARRPESKATNAT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: F2R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB Approx 48kDa band observed in lysates of cell line HeLa (calculated MW of 47.4kDa according to NP_001983.2). Primary incubation 1 hour at room temperature.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PAR1/Thrombin Receptor Antibody

• Cf2r
• CF2RHTR
• coagulation factor II (thrombin) receptor
• Coagulation factor II receptor
• F2R
• HTR
• PAR1
• PAR1PAR-1protease-activated receptor 1
• Protease-Activated Receptor 1
• Thrombin receptor
• ThrR
• TRGPC
• TRproteinase-activated receptor 1

Background

Thrombin is a serine protease that is involved in platelet aggregation and blood coagulation. It is cleaved from its precursor, prothrombin, and converts fibrinogen to fibrin in the final step of the clotting cascade. Thrombin mediates its regulatory effects by activating cell surface receptors. These receptors, including thrombin receptor (also designated PAR-1, for protease-activated receptor-1), PAR-2 and PAR-3 are members of the G-protein coupled receptor family, and share a similiar gene structure. Thrombin cleaves its receptor, releasing a 41 amino acid peptide which acts as a platelet agonist. Upon this activation by thrombin, the thrombin receptors trigger an increase in cytosolic Ca2+ concentration. Unactivated thrombin receptor cycles between the cell surface and an intracellular pool, while activated receptor internalizes rapidly and is degraded in the lysosomes. The human thrombin receptor is also known to be regulated by Sp1 and Sp3 transcription factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAR1/Thrombin Receptor Antibody (NBP1-51936)(1)

Publication using NBP1-51936

• Niessen F, Schaffner F, Furlan-Freguia C et al. Dendritic cell PAR1-S1P3 signalling couples coagulation and inflammation. Nature 2008-04-03 [PMID: 18305483]

Bioinformatics

• Gene Symbol: F2R
• Entrez:
  • 2149(Human)
• Uniprot:
  • NP_001983.2(Human)