PAPSS2 Antibody - BSA Free

Western Blot: PAPSS2 Antibody [NBP1-33726] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: PAPSS2 Antibody [NBP1-33726] - Paraffin-embedded Colon ca, using antibody at 1:200 dilution.

PAPSS2 antibody [N1N3] detects PAPSS2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human hepatocellular carcinoma.PAPSS2 stained by PAPSS2 antibody [N1N3] (NBP1-33726) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Bv, Gp, Rb, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PAPSS2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PAPSS2 Antibody - BSA Free (NBP1-33726) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PAPSS2. The exact sequence is proprietary.
• Predicted Species: Mouse (96%), Rat (96%), Rhesus Macaque (98%), Bovine (93%), Guinea Pig (95%), Rabbit (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAPSS2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PAPSS2 Antibody - BSA Free

• 3'-phosphoadenosine 5'-phosphosulfate synthase 2
• ATP sulfurylase/adenosine 5'-phosphosulfate kinase
• ATPSK2ATP sulfurylase/APS kinase 2
• bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
• bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2
• EC 2.7.1.25
• PAPS synthase 2
• PAPS synthetase 2
• PAPSS 2
• SK 2
• SK2
• Sulfurylase kinase 2,3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 2

Background

Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PAPSS2