PAPSS1 Antibody

Images

 
Western Blot: PAPSS1 Antibody [NBP1-33470] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PAPSS1 Antibody [NBP1-33470] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP1-33470] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.
Western Blot: PAPSS1 Antibody [NBP1-33470] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE, antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.94 mg/ml

Order Details

PAPSS1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PAPSS1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAPSS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (95%) and Xenopus tropicalis (95%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.94 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PAPSS1 Antibody

  • 3'-phosphoadenosine 5'-phosphosulfate synthase 1
  • ATPSK1SK 1
  • bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
  • EC 2.7.1.25
  • PAPS synthase 1
  • PAPSS 1
  • PAPSS3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1
  • SK1
  • Sulfurylase kinase 1

Background

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAPSS1