Pappalysin-2/PAPP-A2 Antibody - BSA Free

Western Blot: Pappalysin-2/PAPP-A2 Antibody [NBP1-76487] - HeLa cell lysate at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry/ Immunofluorescence: Pappalysin-2/PAPP-A2 Antibody - BSA Free [NBP1-76487] - Immunofluorescence of Pappalysin-2/PAPP-A2 in HeLa cells with Pappalysin-2/PAPP-A2 antibody at 20 u/mL.

Immunocytochemistry/ Immunofluorescence: Pappalysin-2/PAPP-A2 Antibody - BSA Free [NBP1-76487] - Immunocytochemistry of Pappalysin-2/PAPP-A2 in HeLa cells with Pappalysin-2/PAPP-A2 antibody at 10 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Pappalysin-2/PAPP-A2 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human PLAC3. The immunogen is located within amino acids 450 - 500 of PLAC3. Amino Acid Squence: VPFRDEKYPRLEVLQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAPPA2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  Pappalysin-2/PAPP-A2 Peptide (NBP1-76487PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Pappalysin-2/PAPP-A2 Antibody - BSA Free

• EC 3.4.24.-
• PAPPA2
• PAPP-A2
• PAPP-A2pappalysin-2
• pappalysin 2
• Pappalysin2
• Pappalysin-2
• PAPPE
• PAPP-E
• PLAC3
• placenta-specific 3
• pregnancy-associated plasma preproprotein-A2
• Pregnancy-associated plasma protein A2
• Pregnancy-associated plasma protein E1

Background

PLAC3 (placenta-specific protein 3), also known as PAPP-A2, is a metalloproteinase that cleaves the insulin-like growth factor-binding protein (IGFBP)-5 in a IGF-independent fashion. It has been suggested that the cleavage of IGFBP-5 and to a lesser extent IGFBP-3 produced in the maternal decidua promotes feto-placental growth. Recent studies have shown elevated levels of PLAC3 in hypertensive disorders of pregnancy including preeclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAPPA2
• Uniprot:
  • NP_064714(Human)