Pancreatic Amylase Alpha Antibody - BSA Free

Western Blot: Pancreatic Amylase Alpha Antibody [NBP1-31724] - Pancreatic Amylase Alpha Antibody Sample (30 ug of whole cell lysate) A: Molt-4 B: Raji 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: Pancreatic Amylase Alpha Antibody [NBP1-31724] - Human ovarian cancer, using alpha amylase 2A(pancreatic) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Amylase immunoreactivity in an FFPE section of canine pancreas. NBP1-31724 was diluted to 2ug/mL and was left on tissue sections for 30min at room temperature. Image from a verified customer review.

Product Discontinued

Pancreatic Amylase Alpha Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Pancreatic Amylase Alpha. The exact sequence is proprietary.
• Localization: Secreted, extracellular space
• Predicted Species: Chimpanzee (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AMY2A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (88%). Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Pancreatic Amylase Alpha Antibody - BSA Free

• AMY2
• AMY2A amylase, alpha 2A (pancreatic)
• AMY2B
• PA
• pancreatic alpha-amylase

Background

Pancreatic Amylase Alpha is a digestive enzyme secreted by the pancreas. It consists of a single polypeptide chain with a molecular weight of 54 kDa. Pancreatic Amylase Alpha occurs in serum at a concentration of about 60 ug/L, from a total amylase concentration in serum of about 120 ug/L. Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Review for Pancreatic Amylase Alpha Antibody (NBP1-31724) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Dog.

reviewed by: Verified Customer IHC-P Dog 07/02/2025

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Pancreas
• Species: Dog
• Lot: 40128

Comments

• Comments: ***Bio-Techne Response: This review reflects a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: AMY2A