pan RhO/RAC/CDC42 Antibody - Azide and BSA Free

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

pan RhO/RAC/CDC42 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Sheep pan RhO/RAC/CDC42 Antibody - Azide and BSA Free (NBP1-46684) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide from RhOA, RhOB, RhOC, RhOF, RhOQ and RAC1, RAC2, RAC3 and CDC42 conjugated to blue carrier protein was used as the antigen.
• Specificity: Specific for RhO family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10-50 ug/ml
  • Immunohistochemistry-Frozen 10-50 ug/ml
  • Immunohistochemistry-Paraffin 10-50 ug/ml
  • Western Blot 10-50 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (1x)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ammonium sulfate precipitation
• Reconstitution Instructions: Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Alternate Names for pan RhO/RAC/CDC42 Antibody - Azide and BSA Free

• h12
• oncogene RHO H12
• ras homolog gene family, member A
• Rho cDNA clone 12
• RHO12
• RhoA
• RHOH12ARH12ARHAAplysia ras-related homolog 12
• small GTP binding protein RhoA
• transforming protein RhoA

Background

Rho belongs to the Ras super family of low molecular weight GTPases. Fifteen Rho proteins have already been characterized and divided into three sub-families; the first includes Rho (A, B, C), the second, Rnd 1-3 and the third which includes Rac 1-3, RhoG, Cdc42Hs, Rho/TTF. TC10 and Chp (1-2). Rho acts as a molecular switch which turns on of off various intracellular signaling pathways such as ACK, PAKs, MEKKs ROCK (3). Rho is active when bound to GTP and inactive when bound to GDP (4). It is also known to participate in many physiological activities including cell migration, adhesion, cytokinesis, proliferation, differentiation and apoptosis and to a greater extend cell transformation (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.