Palladin Antibody (JE66-56)

Western Blot: Palladin Antibody (JE66-56) [NBP3-32709] - Western blot analysis of Palladin on different lysates with Rabbit anti-Palladin antibody (NBP3-32709) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15ug/Lane) Lane 2: SiHa cell lysate (10ug/Lane) Lane 3: A431 cell lysate (15 ug/Lane) Lane 4: SW480 cell lysate (30 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: MDA-MB-231 cell lysate (20 ug/Lane) Lane 8: MDA-MB-468 cell lysate (20 ug/Lane) Lane 9: MCF7 cell lysate (20 ug/Lane) Predicted band size: 151 kDa Observed band size: 110/151 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32709) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu
• Applications: WB
• Clone: JE66-56
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Palladin Antibody (JE66-56) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human Palladin aa 1-100 / 1,383. (Uniprot: Q8WX93)
• Localization: Cytoplasm, cytoskeleton, Cytoplasm, myofibril, sarcomere, Z line.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PALLD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 151 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Palladin Antibody (JE66-56)

• CGI151
• CGI-151
• FLJ22190
• FLJ38193
• KIAA0992FLJ39139
• MYN
• myoneurin
• Palladin
• palladin, cytoskeletal associated protein
• PALLD
• PNCA1
• Sarcoma antigen NY-SAR-77
• SIH002
• SIH002FLJ61376

Background

Palladin is a cytoskeletal protein required for organization of normal actin cytoskeleton. Palladin plays roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. Palladin may function as a scaffolding molecule with the potential to impact both actin polymerization and the assembly of existing actin filaments into higher-order arrays. It binds to proteins that bind to either monomeric or filamentous actin. It localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Palladin's different isoforms may have functional differences. Palladin is also involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PALLD