PALB2 Antibody

Western Blot: PALB2 Antibody [21340002] - HEK293T cells plated at million cell in 10 cm plate overnight before treating with 0.5ug/mL of MMC for 24 hr. Cells were lysed using standard RIPA buffer and 30ug of the samples were used to run the WB. Ab concentration 1:1000. This image was submitted via customer Review.

ELISA: PALB2 Antibody [21340002] - The affinity purified antibody was serially diluted onto anELISA plate coated with a recombinant protein fragment.

Product Discontinued

PALB2 Antibody Summary

• Immunogen: This antibody is specific for the N Terminus Region of the target protein (NM_024675).
• Specificity: This product is specific for Human PALB2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PALB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:1000
• Application Notes: This antibody is useful in ELISA.
• Theoretical MW: 131.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for PALB2 Antibody

• DKFZp667I166
• DKFZp686E1054
• FANCNFLJ21816
• partner and localizer of BRCA2
• PNCA3

Background

PALB2 (partner and localizer of BRCA2) interacts with BRCA2 and promotes BRCA2 localization and stability. PALB2 is also known as FANCN. Bi-allelic mutations in PALB2 result in Fanconi anemia disorder and breast cancer susceptibility. At the cellular level, PALB2 is important to the DNA damage response and chromosome stability and is essential to BRCA2 function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PALB2 Antibody (21340002)(4)

Publications using 21340002

• Toh MR, Low CE, Chong ST et al. Missense PALB2 germline variant disrupts nuclear localization of PALB2 in a patient with breast cancer Fam. Cancer 2020-02-11 [PMID: 32048105]
• Weigelt B, Comino-Mendez I, de Bruijn I et al. Diverse BRCA1 and BRCA2 Reversion Mutations in Circulating Cell-Free DNA of Therapy-Resistant Breast or Ovarian Cancer Clin. Cancer Res. 2017-08-01 [PMID: 28765325] (Human)
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Review for PALB2 Antibody (21340002) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 07/04/2017

Summary

• Application: Western Blot
• Sample Tested: Hek 293T cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: PALB2
• Entrez:
  • 79728(Human)
• Uniprot:
  • Q86YC2(Human)