PAK6 [p Ser165] Antibody

Western Blot: PAK6 [p Ser165] Antibody [NBP3-23266] - Western blots of human PAK6 recombinant protein phosphorylated by ERK2. The blot was exposed to lambda phosphatase (lanes 2 & 6) then probed with anti-PAK6 (N-terminal) (lanes 1-4) or anti-PAK6 (Ser-165) phospho-specific (lanes 5-8). The antibodies were used in the presence of unrelated (lane 3) and PAK6 (N-terminal) (lane 4) peptide or PAK6 (Ser-165) (lane 7) and unrelated phospho-serine (lane 8) peptides, respectively.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PAK6 [p Ser165] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: PAK6 (Ser-165) synthetic peptide (coupled to carrier protein) corresponding to amino acids around serine 165 in human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6. [UniProt# Q9NQU5]
• Modification: p Ser165
• Specificity: This antibody was cross-adsorbed to unphosphorylated PAK6 (Ser-165) peptide then affinity purified using phospho-PAK6 (Ser-165) peptide. The antibody detects a 75 kDa band on SDS-PAGE immunoblots of human PAK6 recombinant protein phosphorylated with ERK2. This reactivity is specifically blocked by pre-incubation with phospho-PAK6 (Ser-165) peptide and also by treatment with lambda phosphatase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAK6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for PAK6 [p Ser165] Antibody

• EC 2.7.11
• p21 protein (Cdc42/Rac)-activated kinase 6
• p21(CDKN1A)-activated kinase 6
• p21-activated kinase 6
• p21-activated protein kinase 6
• PAK-5
• PAK5EC 2.7.11.1
• PAK6
• PAK-6
• serine/threonine-protein kinase PAK 6

Background

PAK6, a novel AR-interacting protein, shares a high degree of sequence similarity with other p21-activated kinases (PAK). PAK6 co-translocates into the nucleus with AR in response to androgen. In addition, AR and ER alpha transcriptional activites were inhibited by PAK6 in transient transfections with episomal and integrated reporter genes. PAK, which is highly expressed in testis and prostate tissues, could contribute to the effects of tamoxifen in breast cancer and in other tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAK6