Recombinant Human PAK4 Protein

Product Discontinued

Recombinant Human PAK4 Protein Summary

• Description: Recombinant protein for Human PAK4
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(O96013)

• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: PAK4
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 83 nmol phosphate incorporated into the PKB-sub peptide (CKRPRAASFAE) per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
• Concentration: 0.1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human PAK4 Protein

• EC 2.7.11
• EC 2.7.11.1
• KIAA1142
• p21 protein (Cdc42/Rac)-activated kinase 4
• p21(CDKN1A)-activated kinase 4
• p21-activated kinase 4
• PAK4
• PAK-4
• protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
• serine/threonine-protein kinase PAK 4

Background

PAK4 is a recently identified member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, cdc 42 and Rac. PAK4 belongs to the Group II PAKs which also includes PAK5 and PAK6 while Group I PAKs comprise of PAK1, PAK2 and PAK3. PAK4 differs from other members of the PAK family both in sequence and function. PAK4 has been shown to regulate cell morphology and cytoskeletal organization in mammalian cells. PAK4 regulates the activity of LIM kinase 1 which in turn phosphorylates Cofilin leading to cytoskeletal changes. PAK4 can protect cells from apoptosis in response to several different types of stimuli by inhibiting the pro-apoptotic proteins Bad and Caspase 8. PAK4 has been shown to associate with and mediate the downstream signaling of the keratinocyte growth factor receptor. In addition, PAK4 interacts with the Beta 5 integrins and regulates cell migration during engagement of thealphavBeta 5 integrin receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PAK4