Human PAK4 ELISA Kit (Colorimetric)

ELISA: Human PAK4 ELISA Kit (Colorimetric) [NBP2-60591]

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture
• Standard Curve Range: 0.313 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.1 ng/ml (example only; lot dependent)

Human PAK4 ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.313 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.1 ng/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.40% (example only; lot dependent)
• Intra-Assay: CV% < 4.20% (example only; lot dependent)
• Spike Recovery: 96.00% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PAK4

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human PAK4 ELISA Kit (Colorimetric)

• EC 2.7.11
• EC 2.7.11.1
• KIAA1142
• p21 protein (Cdc42/Rac)-activated kinase 4
• p21(CDKN1A)-activated kinase 4
• p21-activated kinase 4
• PAK4
• PAK-4
• protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
• serine/threonine-protein kinase PAK 4

Background

p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes, including the cell cycle, dynamics of the cytoskeleton, apoptosis, oncogenic transformation, and transcription. All PAK family members are characterized by the presence of p21-binding domain. p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, and lipids, which stimulate autophosphorylation and phosphorylation of exogenous substrates. Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo. Phosphospecific antibodies directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42. Current data strongly implicates PAK-4 in oncogenesis. PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PAK4