Human PAK4 ELISA Kit (Colorimetric)

ELISA: Human PAK4 ELISA Kit (Colorimetric) [NBP2-60591]

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture
• Standard Curve Range: 0.313 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.1 ng/ml (example only; lot dependent)

Human PAK4 ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.313 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.1 ng/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.40% (example only; lot dependent)
• Intra-Assay: CV% < 4.20% (example only; lot dependent)
• Spike Recovery: 96.00% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PAK4

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human PAK4 ELISA Kit (Colorimetric)

• EC 2.7.11
• EC 2.7.11.1
• KIAA1142
• p21 protein (Cdc42/Rac)-activated kinase 4
• p21(CDKN1A)-activated kinase 4
• p21-activated kinase 4
• PAK4
• PAK-4
• protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
• serine/threonine-protein kinase PAK 4

Background

p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes, including the cell cycle, dynamics of the cytoskeleton, apoptosis, oncogenic transformation, and transcription. All PAK family members are characterized by the presence of p21-binding domain. p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, and lipids, which stimulate autophosphorylation and phosphorylation of exogenous substrates. Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo. Phosphospecific antibodies directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42. Current data strongly implicates PAK-4 in oncogenesis. PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for PAK4 ELISA Kit (NBP2-60591). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PAK4