PAK2 Antibody (JB41-84)

Western Blot: PAK2 Antibody (JB41-84) [NBP2-75607] - Analysis of PAK2 on mouse thymus tissue (1) and MCF-7 cell (2) lysate using anti-PAK2 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: PAK2 Antibody (JB41-84) [NBP2-75607] - Staining PAK2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PAK2 Antibody (JB41-84) [NBP2-75607] - Analysis of paraffin-embedded rat kidney tissue using anti-PAK2 antibody. Counter stained with hematoxylin.

Flow Cytometry: PAK2 Antibody (JB41-84) [NBP2-75607] - Analysis of A431 cells with PAK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PAK2 Antibody (JB41-84) [NBP2-75607] - Staining PAK2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PAK2 Antibody (JB41-84) [NBP2-75607] - Analysis of paraffin-embedded human colon cancer tissue using anti-PAK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PAK2 Antibody (JB41-84) [NBP2-75607] - Analysis of paraffin-embedded human liver tissue using anti-PAK2 antibody. Counter stained with hematoxylin.

Product Discontinued

PAK2 Antibody (JB41-84) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within N-terminal Human PAK2. (SwissProt: Q13177 Human; SwissProt: Q8CIN4 Mouse; SwissProt: Q64303 Rat)
• Localization: Cytoplasm, Membrane, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PAK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:1000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PAK2 Antibody (JB41-84)

• EC 2.7.11
• gamma-PAK
• p21 (CDKN1A)-activated kinase 2
• p21 protein (Cdc42/Rac)-activated kinase 2
• p21-activated kinase 2
• p58
• PAK2
• PAK-2
• PAK65
• PAK65EC 2.7.11.1
• PAKgamma
• S6/H4 Kinase
• serine/threonine-protein kinase PAK 2

Background

p21-activated kinase 2 (PAK2) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). All six PAKs play an important role in diverse cellular processes, including cytoskeletal dynamics, growth/apoptotic signal transduction through MAP kinases, and regulation of transcription factors (4). The ubiquitous PAK-2 is unique among the PAK isoforms because it is also activated through proteolytic cleavage by caspases or caspase-like proteases (5). Full-length PAK-2 is localized in the cytoplasm, whereas the proteolytic PAK-2p34 fragment translocates to the nucleus. Activation of full-length PAK-2 stimulates cell survival, whereas proteolytic activation of PAK-2p34 is involved in programmed cell death (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAK2