PAK1/2/3 [p Ser141] Antibody

Product Discontinued

PAK1/2/3 [p Ser141] Antibody Summary

• Immunogen: Chemically synthesized phosphopeptide derived from a region of human PAK2 that contains serine 141. The sequence is conserved in rat and rabbit.
• Modification: p Ser141
• Localization: Cytoplasm. Recruited to focal adhesions upon activation
• Specificity: PAK 1 + 2 + 3 Phosphospecific [Ser141]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot
• Application Notes: Western Blot Predicted molecular weight 65 kDa.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with Rabbit and Rat due to sequence identity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PAK1/2/3 [p Ser141] Antibody

• alpha-PAK
• EC 2.7.11
• EC 2.7.11.1
• MGC130000
• MGC130001
• p21 protein (Cdc42/Rac)-activated kinase 1
• p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
• p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
• p21-activated kinase 1
• p65-PAK
• PAK-1
• PAKalpha
• serine/threonine-protein kinase PAK 1
• STE20 homolog, yeast

Background

P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAK1
• Entrez:
  • 5058(Human)