PAFAH1B2 Antibody - Azide and BSA Free

Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-PAFAH1B2 )

ELISA: PAFAH1B2 Antibody [NB120-15875] - Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antibody (1 ug/ml) and serial dilutions of antigen. ( anti-PAFAH1B2 )

Product Discontinued

PAFAH1B2 Antibody - Azide and BSA Free Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 1-229 of PAFAH1B2.
• Localization: Cytoplasmic
• Specificity: PAFAH1B2
• Clonality: Polyclonal
• Host: Chicken
• Gene: PAFAH1B2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:200 - 1:500
  • Western Blot 1:200 - 1:500
• Application Notes: WB: Use at a dilution of 1/2000. Predicted molecular weight: 25 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
• Control:

  PAFAH1B2 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human, Mouse and Rat.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for PAFAH1B2 Antibody - Azide and BSA Free

• EC 3.1.1.47
• intracellular platelet-activating factor acetylhydrolase alpha 2 subunit
• PAF acetylhydrolase 30 kDa subunit
• PAF-AH 30 kDa subunit
• PAFAH subunit beta
• PAF-AH subunit beta
• PAF-AH1b alpha 2 subunit
• PAFAHB
• platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa)
• platelet-activating factor acetylhydrolase IB subunit beta
• platelet-activating factor acetylhydrolase, isoform Ib, beta subunit 30kDa
• platelet-activating factor acetylhydrolase, isoform Ib, subunit 2 (30kDa)

Background

Platelet-activating factor (PAF) is a biologically active phospholipid with diverse biologic effects. PAF is degraded to inactive products by hydrolysis of the acetyl group at the sn2 position to produce the biologically inactive products LYSO PAF and acetate. This reaction is catalyzed by PAF acetylhydrolase (PAFAH). The various monomeric and multimeric forms of the enzyme are composed of alpha, beta, and gamma PAFAH subunits. The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAFAH1B2
• Entrez:
  • 5049(Human)
  • 18475(Mouse)
  • 64189(Rat)