PAFAH1B2 Antibody

Images

 
Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-PAFAH1B2 )
ELISA: PAFAH1B2 Antibody [NB120-15875] - Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antibody (1 ug/ml) and serial dilutions of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PAFAH1B2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 1-229 of PAFAH1B2.
Localization
Cytoplasmic
Specificity
PAFAH1B2
Clonality
Polyclonal
Host
Chicken
Gene
PAFAH1B2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
WB: Use at a dilution of 1/2000. Predicted molecular weight: 25 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Control
PAFAH1B2 Lysate (NBL1-14065)

Reactivity Notes

Cross-reacts with Human, Mouse and Rat.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PAFAH1B2 Antibody

  • EC 3.1.1.47
  • intracellular platelet-activating factor acetylhydrolase alpha 2 subunit
  • PAF acetylhydrolase 30 kDa subunit
  • PAF-AH 30 kDa subunit
  • PAFAH subunit beta
  • PAF-AH subunit beta
  • PAF-AH1b alpha 2 subunit
  • PAFAHB
  • platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa)
  • platelet-activating factor acetylhydrolase IB subunit beta
  • platelet-activating factor acetylhydrolase, isoform Ib, beta subunit 30kDa
  • platelet-activating factor acetylhydrolase, isoform Ib, subunit 2 (30kDa)

Background

Platelet-activating factor (PAF) is a biologically active phospholipid with diverse biologic effects. PAF is degraded to inactive products by hydrolysis of the acetyl group at the sn2 position to produce the biologically inactive products LYSO PAF and acetate. This reaction is catalyzed by PAF acetylhydrolase (PAFAH). The various monomeric and multimeric forms of the enzyme are composed of alpha, beta, and gamma PAFAH subunits. The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAFAH1B2
Entrez