PAF1 Antibody

Images

 
Western Blot: PAF1 Antibody [NB600-273] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPaf1 antibody ...read more
Independent Antibodies: Immunoprecipitation: PAF1 Antibody [NB600-273] - Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Pm-OrSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

PAF1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 75 and 125 of human RNA Polymerase Associated Factor using the numbering given in TrEMBL entry Q9NUU9 (GeneID 54623).
Predicted Species
Rat (100%), Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAF1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000- 1:15000
Publications
Read Publications using NB600-273.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PAF1 Antibody

  • F23149_1
  • FLJ11123
  • Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
  • Pancreatic differentiation protein 2
  • PD2hPAF1
  • RNA polymerase II-associated factor 1 homolog

Background

Paf1 has been shown to form a complex with Rtf1, Ctr9, Cdc73, and Leo1 in yeast that may mediate the interaction between the elongation factor Spt16/Pob3 (FACT in humans) and RNA polymerase II. This mediation suggests that this complex may have an important role in transcription elongation, thereby suggesting that Paf1, Rtf1, Ctr9, Cdc73 and Leo1 may be elongation factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PAF1 Antibody (NB600-273)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PAF1 Antibody (NB600-273). (Showing 1 - 1 of 1 FAQs).

  1. We used a PAF1 Antibody (NB600-273) (lot no. A1) and I'm wondering if you can let me know the molar concentration (eg. 1M) and the mass concentration (eg. 1mg/ml) of this particular antibody?
    • This antibody is an immunogen affinity purified whole IgG and because the molecular weight of IgG is 150kDa, the molar concentration of this antibody would be 1.33uM.

Secondary Antibodies

 

Isotype Controls

Additional PAF1 Products

Array NB600-273

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Bioinformatics

Gene Symbol PAF1
Entrez
Uniprot