PAF1 Antibody

Western Blot: PAF1 Antibody [NB600-273] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPaf1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: PAF1 Antibody [NB600-273] - Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Paf1 antibody NB600-273 used for IP at 3 ug per reaction. Paf1 was also immunoprecipitated by rabbit anti-Paf1 antibody NB600-274. For blotting immunoprecipitated Paf1, NB600-273 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu, Rt, Bv, Pm-Or
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PAF1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 75 and 125 of human RNA Polymerase Associated Factor using the numbering given in TrEMBL entry Q9NUU9 (GeneID 54623).
• Predicted Species: Rat (100%), Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000- 1:15000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PAF1 Antibody

• F23149_1
• FLJ11123
• Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
• Pancreatic differentiation protein 2
• PD2hPAF1
• RNA polymerase II-associated factor 1 homolog

Background

Paf1 has been shown to form a complex with Rtf1, Ctr9, Cdc73, and Leo1 in yeast that may mediate the interaction between the elongation factor Spt16/Pob3 (FACT in humans) and RNA polymerase II. This mediation suggests that this complex may have an important role in transcription elongation, thereby suggesting that Paf1, Rtf1, Ctr9, Cdc73 and Leo1 may be elongation factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAF1 Antibody (NB600-273)(5)

Publications using NB600-273

• Langenbacher AD, Nguyen CT, Cavanaugh AM et al. The PAF1 complex differentially regulates cardiomyocyte specification. Dev Biol 2011-05-01 [PMID: 21338598]
• Jurynec MJ, Grunwald DJ. SHIP2, a factor associated with diet-induced obesity and insulin sensitivity, attenuates FGF signaling in vivo. Dis Model Mech 2010-11-01 [PMID: 20616095]
• Panicker LM, Zhang JH, Dagur PK et al. Defective nucleolar localization and dominant interfering properties of a parafibromin L95P missense mutant causing the hyperparathyroidism-jaw tumor syndrome. Endocr Relat Cancer 2010-05-01 [PMID: 20304979]
• Youn MY, Yoo HS, Kim MJ et al. hCTR9, a component of Paf1 complex, participates in the transcription of interleukin 6-responsive genes through regulation of STAT3-DNA interactions. J Biol Chem 2007-11-01 [PMID: 17911113]
• Lin L, Czapiga M, Nini L et al. Nuclear localization of the parafibromin tumor suppressor protein implicated in the hyperparathyroidism-jaw tumor syndrome enhances its proapoptotic function. Mol Cancer Res 2007-02-01 [PMID: 17314275]

FAQs for PAF1 Antibody (NB600-273). (Showing 1 - 1 of 1 FAQs).

1. We used a PAF1 Antibody (NB600-273) (lot no. A1) and I'm wondering if you can let me know the molar concentration (eg. 1M) and the mass concentration (eg. 1mg/ml) of this particular antibody?
   • This antibody is an immunogen affinity purified whole IgG and because the molecular weight of IgG is 150kDa, the molar concentration of this antibody would be 1.33uM.

Bioinformatics

• Gene Symbol: PAF1
• Entrez:
  • 54623(Human)
• Uniprot:
  • Q9NUU9(Human)