PACSIN3 Antibody (S04-1F1)

Western Blot: PACSIN3 Antibody (S04-1F1) [NBP3-19817] - Western blot detection of PACSIN3 in Hela, A549 cell lysates using NBP3-19817 (1:1000 diluted). Predicted band size: 49kDa. Observed band size: 49kDa.

Immunocytochemistry/Immunofluorescence: PACSIN3 Antibody (S04-1F1) [NBP3-19817] - Immunocytochemistry of PACSIN3 (green) in Hela using NBP3-19817 at dilution 1/2, and DAPI (blue)

Immunohistochemistry-Paraffin: PACSIN3 Antibody (S04-1F1) [NBP3-19817] - Immunohistochemistry of PACSIN3 in paraffin-embedded Human colon cancer tissue using NBP3-19817 at dilution 1/50

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: S04-1F1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

PACSIN3 Antibody (S04-1F1) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human PACSIN3 (Uniprot # Q9UKS6)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PACSIN3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/20
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000-1/5000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for PACSIN3 Antibody (S04-1F1)

• Endophilin I
• FLJ17861
• protein kinase C and casein kinase substrate in neurons 3
• protein kinase C and casein kinase substrate in neurons protein 3
• SDPIII
• SH3 domain-containing protein 6511
• syndapin III

Background

PACSIN3 (protein kinase C and casein kinase substrate in neurons protein 3) is a member of the PACSIN family, a group of adaptor protein that regulate vesicle trafficking via cytoskeletal alterations. PACSIN3 is primarily expressed in skeletal muscle and heart and has been shown to be specifically involved in the trafficking of GLUT 1 in adipocytes. PACSIN3 is also known as SH3 domain-containing 6511, syndapin III, and SDPIII.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PACSIN3