PA28 Activator alpha Subunit/PSME1 Antibody

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Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NB100-59751] - Analysis of PSME1 (isoform 1) using this antibody at 0.3 ug/ml in Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PA28 Activator alpha Subunit/PSME1 Antibody Summary

Immunogen
Peptide with sequence C-KNFEKLKKPRGETK corresponding to C-Terminus according to NP_006254.1.
Epitope
KNFEKLKKPRGETK
Specificity
This antibody is expected to recognise isoform 1 (NP_006254.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PSME1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 30 kDa band observed in human lymph node, spleen, tonsil and peripheral blood mononuceocyte lysates (calculated MW of 28.7 kDa band according to NP_006254.1).
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Blood Leukocyte Lysate (NB820-59174)
PA28 Activator alpha Subunit/PSME1 Lysate (NBL1-14907)
Publications
Read Publication using NB100-59751.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PA28 Activator alpha Subunit/PSME1 Antibody

  • 11S regulator complex alpha subunit
  • 11S Regulator Complex Subunit alpha
  • 29-kD MCP activator subunit
  • IFI5111
  • IFI5111MGC8628
  • IGUP I-5111
  • Interferon gamma up-regulated I-5111 protein
  • interferon-gamma IEF SSP 5111
  • interferon-gamma-inducible protein 5111
  • PA28 Activator alpha Subunit
  • PA28A
  • PA28alphaActivator of multicatalytic protease subunit 1
  • proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
  • Proteasome activator 28 subunit alpha
  • proteasome activator complex subunit 1
  • proteasome activator subunit-1
  • PSME1
  • REG alpha
  • REGalpha
  • REG-alpha

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSME1
Entrez
Uniprot