| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83121-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26364-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59434-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90298-50ul | |||
![Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP1-83121] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-Western-Blot-NBP1-83121-img0007.jpg "Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP1-83121] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: PA28 Activator alpha Subunit/PSME1 Antibody [NBP1-83121] - Staining of human tonsil shows strong cytoplasmic positivity in germinal and non-germinal center cells.](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-Immunohistochemistry-Paraffin-NBP1-83121-img0006.jpg)
![Immunohistochemistry: PA28 Activator alpha Subunit/PSME1 Antibody (29D5) [NBP3-26364] - Image of PA28 Activator alpha Subunit/PSME1 Antibody (29D5) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26364_rabbit-pa28-activator-alpha-subunit-psme1-mab-29d5-262202414225213.jpg "Immunohistochemistry: PA28 Activator alpha Subunit/PSME1 Antibody (29D5) [NBP3-26364] - Image of PA28 Activator alpha Subunit/PSME1 Antibody (29D5) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PA28 Activator alpha Subunit/PSME1 Antibody (29D5) [NBP3-26364] - Image of PA28 Activator alpha Subunit/PSME1 Antibody (29D5) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26364_rabbit-pa28-activator-alpha-subunit-psme1-mab-29d5-27220248274917.jpg)
![Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (29D5) [NBP3-26364] - Positive Western Blot detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, Ntera-2 whole cell lysate, U251 whole cell lysate. All lanes: PA28 Activator alpha Subunit/PSME1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 27 kDa Observed band size: 28-30, 55-72 kDa](http://images.novusbio.com/fullsize/nbp3-26364_rabbit-pa28-activator-alpha-subunit-psme1-mab-29d5-28220241141365.jpg)
![Immunocytochemistry/Immunofluorescence: PA28 Activator alpha Subunit/PSME1 Antibody (29D5) [NBP3-26364] - Staining of HepG2 cell with PA28 Activator alpha Subunit/PSME1 Antibody (29D5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 518-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26364_rabbit-pa28-activator-alpha-subunit-psme1-mab-29d5-282202412493617.jpg)
![Flow Cytometry: PA28 Activator alpha Subunit/PSME1 Antibody (29D5) [NBP3-26364] - Overlay Peak curve showing HepG2 cells stained with PA28 Activator alpha Subunit/PSME1 Antibody (29D5) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26364_rabbit-pa28-activator-alpha-subunit-psme1-mab-29d5-282202414183652.jpg)
![Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [H00005720-B01P] - Analysis of PSME1 expression in A-431.](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-Western-Blot-H00005720-B01P-img0004.jpg "Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [H00005720-B01P] - Analysis of PSME1 expression in A-431.")
![Immunocytochemistry/Immunofluorescence: PA28 Activator alpha Subunit/PSME1 Antibody [H00005720-B01P] - Analysis of purified antibody to PSME1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-Immunocytochemistry-Immunofluorescence-H00005720-B01P-img0003.jpg)
![Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [H00005720-B01P] - Analysis of PSME1 expression in transfected 293T cell line by PSME1 polyclonal antibody. Lane 1: PSME1 transfected lysate(27.5 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-Western-Blot-H00005720-B01P-img0005.jpg)
![Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19991] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-Western-Blot-NBP2-19991-img0002.jpg "Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19991] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE gel, diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19991] - H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PSME1 protein stained by PSME1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19991-img0003.jpg)
![Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NB100-59751] - Analysis of PSME1 (isoform 1) using this antibody at 0.3 ug/ml in Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer).](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-Western-Blot-NB100-59751-img0004.jpg "Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NB100-59751] - Analysis of PSME1 (isoform 1) using this antibody at 0.3 ug/ml in Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer).")
![Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (12H3) [NBP2-59434] - Lane 1: HeLa cell lysates, Lane 2: LnCaP cell lysates, Lane 3: A549 cell lysates, Lane 4: 293T cell lysates, Lane 5: Jurkat cell lysates, Lane 6: Raji cell lysates, Lane 7: A431 cell lysates](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-12H3-Western-Blot-NBP2-59434-img0001.jpg "Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (12H3) [NBP2-59434] - Lane 1: HeLa cell lysates, Lane 2: LnCaP cell lysates, Lane 3: A549 cell lysates, Lane 4: 293T cell lysates, Lane 5: Jurkat cell lysates, Lane 6: Raji cell lysates, Lane 7: A431 cell lysates")
![Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (016) [NBP2-90298] - Lane A: HL60 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: MCF7 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-016-Western-Blot-NBP2-90298-img0001.jpg "Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (016) [NBP2-90298] - Lane A: HL60 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: MCF7 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa")
![Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (6H7X3) [NBP3-16543] - Western blot analysis of extracts of various cell lines, using PA28 Activator alpha Subunit/PSME1 Rabbit mAb (NBP3-16543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-6H7X3-Western-Blot-NBP3-16543-img0001.jpg "Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (6H7X3) [NBP3-16543] - Western blot analysis of extracts of various cell lines, using PA28 Activator alpha Subunit/PSME1 Rabbit mAb (NBP3-16543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunoprecipitation:PA28 Activator alpha Subunit/PSME1 AntibodyNBP3-30556] -Detection of human PSME1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody NBP3-30556 (lot NBP3-30556-1) used for IP at 6 µg/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, NBP3-30556 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30556_rabbit-pa28-activator-alpha-subunit-psme1-pab-3012202417101312.jpg "Immunoprecipitation:PA28 Activator alpha Subunit/PSME1 AntibodyNBP3-30556] -Detection of human PSME1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody NBP3-30556 (lot NBP3-30556-1) used for IP at 6 µg/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, NBP3-30556 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19992] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/PA28-Activator-alpha-Subunit-PSME1-Antibody-Western-Blot-NBP2-19992-img0002.jpg "Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19992] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:5000.")
![Western Blot: PSME1 Overexpression Lysate (Adult Normal) [NBL1-14907] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PSME1.](http://images.novusbio.com/fullsize/PSME1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14907-img0002.jpg "Western Blot: PSME1 Overexpression Lysate (Adult Normal) [NBL1-14907] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PSME1.")