p97/VCP Antibody (Hs-14) [PE]

Product Discontinued

p97/VCP Antibody (Hs-14) [PE] Summary

• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Specificity: The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCP
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Size Exclusion Chromatography

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for p97/VCP Antibody (Hs-14) [PE]

• 15S Mg(2+)-ATPase p97 subunit
• IBMPFD
• IBMPFDMGC8560
• MGC131997
• p97
• TER ATPase
• TERA
• transitional endoplasmic reticulum ATPase
• valosin containing protein
• Valosin-containing proteinMGC148092
• VCP
• yeast Cdc48p homolog

Background

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Intra Acrosomal Protein Antibody (NB500-455PE)(2)

Publications using NB500-455PE

• Elemento JK, Cohen PE Dynamic transcriptome profiles within spermatogonial and 2 spermatocyte populations during postnatal testis maturation 3 revealed by single-cell sequencing biorxiv.org (Mouse)
• Elemento JK, Cohen PE Dynamic transcriptome profiles within spermatogonial and 2 spermatocyte populations during postnatal testis maturation 3 revealed by single-cell sequencing PLoS Genet 2019-03-21 [PMID: 30893341]

Bioinformatics

• Gene Symbol: VCP
• Entrez:
  • 56(Human)
  • 11451(Mouse)