p73 Antibody - BSA Free

Western Blot: p73 Antibody [NB100-420] - Whole cell lysate (50 ug) from HEK293T, HeLa, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p73 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Western Blot: p73 Antibody [NB100-420] - Detection of Human p73 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-p73 antibody NB100-420 used for WB at 1 ug/ml (A and B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

p73 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit p73 Antibody - BSA Free (NB100-420) is a polyclonal antibody validated for use in WB and IP. Anti-p73 Antibody: Cited in 13 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide mapping to a region between residues 1 and 62 of human tumor protein p73 using the numbering given in Swiss-Prot entry O15350 (GeneID 7161).
• Specificity: The epitope is found in isoforms alpha, beta, gamma, delta, epsilon, zeta, and kappa of human p73. The antibody is not expected to react with deltaNp73.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP73
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg of lysate
  • Western Blot 1:2000 - 1:10000
• Control:

  p73 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for p73 Antibody - BSA Free

• p53-related protein
• P73p53-like transcription factor
• TA p73
• TAp73
• tumor protein p73

Background

p73 maps to chromosome 1p36, a region frequently deleted in certain tumors and believed to contain tumor suppressor genes. In addition, p73 shares considerable homology with the tumor suppressor p53. This homology is as follows: amino acids 1-45 (transactivation domain) 29%; residues 113-290 (DNA-binding region) 63%; and residues 319-363 (oligomerization region) 38%. This strong homology suggests functional similarities as seen by p73 transactivating the p53 target gene p21WAF1 and apoptosis when overexpressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p73 Antibody (NB100-420)(13)

Publications using NB100-420

• Ruhul Amin AR, Thakur VS, Gupta K et al. N-(phosphonacetyl)-L-aspartate induces TAp73-dependent apoptosis by modulating multiple Bcl-2 proteins: potential for cancer therapy. Oncogene 2012-03-01 [PMID: 22430213]
• Tebbi A, Guittet O, Cottet MH et al. TAp73 induction by nitric oxide: regulation by checkpoint kinase 1 (CHK1) and protection against apoptosis. J Biol Chem. 2011-03-11 [PMID: 21212274]
• Barton CE, Johnson KN, Mays DM et al. Novel p63 target genes involved in paracrine signaling and keratinocyte differentiation. Cell Death Dis 2010-01-01 [PMID: 21151771]
• Rana S, Gupta K, Gomez J et al. Securinine induces p73-dependent apoptosis preferentially in p53-deficient colon cancer cells. FASEB J 2010-06-01 [PMID: 20133503]
• Zhang J, Chen X, Kent MS et al. Establishment of a dog model for the p53 family pathway and identification of a novel isoform of p21 cyclin-dependent kinase inhibitor. Mol Cancer Res 2009-01-01 [PMID: 19147538]
• Dar AA, Belkhiri A, Ecsedy J et al. Aurora kinase A inhibition leads to p73-dependent apoptosis in p53-deficient cancer cells. Cancer Res 2008-11-01 [PMID: 18974145]
• Levy D, Reuven N, Shaul Y et al. A regulatory circuit controlling Itch-mediated p73 degradation by Runx. J Biol Chem 2008-10-01 [PMID: 18701449]
• Rosenbluth Jennifer M, Mays Deborah J, Pino Maria F et al. A gene signature-based approach identifies mTOR as a regulator of p73. Mol Cell Biol. 2008-10-01 [PMID: 18678646]
• Kravchenko JE, Ilyinskaya GV, Komarov PG et al. Small-molecule RETRA suppresses mutant p53-bearing cancer cells through a p73-dependent salvage pathway. Proc Natl Acad Sci U S A 2008-04-01 [PMID: 18424558]
• Kommagani R, Payal V, Kadakia MP et al. Differential regulation of vitamin D receptor (VDR) by the p53 Family: p73-dependent induction of VDR upon DNA damage. J Biol Chem 2007-10-01 [PMID: 17716971]
• Amin AR, Thakur VS, Paul RK et al. SHP-2 tyrosine phosphatase inhibits p73-dependent apoptosis and expression of a subset of p53 target genes induced by EGCG. Proc Natl Acad Sci U S A 2007-03-01 [PMID: 17369354]
• Zhang J, Chen X. DeltaNp73 modulates nerve growth factor-mediated neuronal differentiation through repression of TrkA. Mol Cell Biol 2007-05-01 [PMID: 17353261]
• Levy D, Adamovich Y, Reuven N et al. The Yes-associated protein 1 stabilizes p73 by preventing Itch-mediated ubiquitination of p73. Cell Death Differ 2007-04-01 [PMID: 17110958]

Bioinformatics

• Gene Symbol: TP73
• Entrez:
  • 7161(Human)
• Uniprot:
  • O15350(Human)