p73 Antibody (5B1288) [DyLight 488]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
5B1288
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

p73 Antibody (5B1288) [DyLight 488] Summary

Immunogen
This antibody was raised against full-length human p73. The epitope is thought to lie around the center of the molecule (NP_005418).
Specificity
It reacts with alpha, beta, gamma and delta isoforms of mouse and human p73 as well as the dominant negative p73 (Costanzo, et al, 2002). Because of its reactivity pattern it may be regarded as anti-pan p73. Immunoprecipitation details can be found in Sayan et al (2005). NB100-56674 has been shown to recongize all the known alternative splicing variants of human and mouse p73 (Costanzo et al, 2002). The antibody does not cross react with p53.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TP73
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
Reported use for Chromatin Immunoprecipitation assay (See accardi et al); Immunocytochemistry/Immunofluorescence and Immunoprecipitation (see Sayan et al)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for p73 Antibody (5B1288) [DyLight 488]

  • p53-related protein
  • P73p53-like transcription factor
  • TA p73
  • TAp73
  • tumor protein p73

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p73 Antibody (NB100-56674G) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-56674B
DyLight 350 Labeled NB100-56674UV
DyLight 405 Labeled NB100-56674V
DyLight 550 Labeled NB100-56674R
DyLight 650 Labeled NB100-56674C
DyLight 680 Labeled NB100-56674FR
DyLight 755 Labeled NB100-56674IR
HRP Labeled NB100-56674H

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Blogs on p73.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

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Bioinformatics

Gene Symbol TP73