p73 Antibody (5B1288)

Images

 
Western Blot: p73 Antibody (5B1288) [NB100-56674] - Analysis of p73 in HeLa cell lysate (Cat no. NBP2-25045) using this antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: p73 Antibody (5B1288) [NB100-56674] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast cancer section using 10ug/ml conc. of p73 antibody (clone 5B1288) on a Bond Rx ...read more
Western Blot: p73 Antibody (5B1288) [NB100-56674] - Analysis of p73 in transfected cell lysate using this antibody.
Immunohistochemistry-Paraffin: p73 Antibody (5B1288) [NB100-56674] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast cancer section using 10ug/ml conc. of p73 antibody (clone 5B1288) on a Bond Rx ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clone
5B1288
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

p73 Antibody (5B1288) Summary

Immunogen
This antibody was raised against full-length human p73. The epitope is thought to lie around the center of the molecule (NP_005418).
Specificity
It reacts with alpha, beta, gamma and delta isoforms of mouse and human p73 as well as the dominant negative p73 (Costanzo, et al, 2002). Because of its reactivity pattern it may be regarded as anti-pan p73. Immunoprecipitation details can be found in Sayan et al (2005). NB100-56674 has been shown to recongize all the known alternative splicing variants of human and mouse p73 (Costanzo et al, 2002). The antibody does not cross react with p53.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TP73
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Chromatin Immunoprecipitation 1:20-1:1000
  • Immunocytochemistry/Immunofluorescence 1:20-1:2000
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Immunoprecipitation 1:20-1:1000
Application Notes
Reported use for Chromatin Immunoprecipitation assay (See accardi et al); Immunocytochemistry/Immunofluorescence and Immunoprecipitation (see Sayan et al). Use in Immunohistochemistry reported in scientific literature (PMID 19816568) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
p73 Lysate (NBL1-17209)
Publications
Read Publications using
NB100-56674 in the following applications:

  • 1 publication
  • 1 publication
  • 1 publication
  • IHC
    1 publication
  • IP
    2 publications
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Notes

The clone has also been referred to as 1288 in the literature. Endogenous expression of p73 has been detected in a variety of cell types; please refer to Product Citations for details regarding culture and treatment conditions.

Alternate Names for p73 Antibody (5B1288)

  • p53-related protein
  • P73p53-like transcription factor
  • TA p73
  • TAp73
  • tumor protein p73

Background

p73 was identified as a long-lost cousin of tumor suppressor protein, p53. p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein. Tumor BioMarker: Vella et al (2003) found p73 to be upregulated in a significant fraction of anaplastic thyroid cancers, whereas p73 was not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for p73 Antibody (NB100-56674)(29)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ChIP, CoIP, ICC/IF, IHC, IP, WB.


Filter By Application
ChIP
(1)
CoIP
(1)
ICC/IF
(1)
IHC
(1)
IP
(2)
WB
(7)
All Applications
Filter By Species
Human
(7)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB100-56674 Applications Species
Ng KY, Chan LH, Chai S et al. TP53INP1 down-regulation activates a p73-dependent DUSP10/ERK signaling pathway to promote metastasis of hepatocellular carcinoma Cancer Res. 2017 Jul 03 [PMID: 28674078] (Human) Human
Venkatanarayan A, Raulji P, Norton W et al. IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo Nature et al. 2014 Nov 17 [PMID: 25409149] (WB, ChIP, Mouse) WB, ChIP Mouse
Lu H, Yan C, Quan XX et al. CK2 Phosphorylates and Inhibits TAp73 Tumor Suppressor Function to Promote Expression of Cancer Stem Cell Genes and Phenotype in Head and Neck Cancer. Neoplasia. 2014 Oct 01 [PMID: 25379016] (CoIP, WB, Human) CoIP, WB Human
Guerrieri F, Piconese S, Lacoste C et al. The sodium/iodide symporter NIS is a transcriptional target of the p53-family members in liver cancer cells. Cell Death Dis. 2013 [PMID: 24052075] (Human) Human
Du W, Jiang P, Mancuso A et al. TAp73 enhances the pentose phosphate pathway and supports cell proliferation. Nat Cell Biol. 2013 Aug [PMID: 23811687]
King KE, Reddi DM, Ponnamperuma RM et al. Dysregulated deltaNp63alpha negatively regulates the maspin promoter in keratinocytes via blocking endogenous p73 binding Mol Carcinog 2013 Mar 8 [PMID: 23475637] (IP, Mouse) IP Mouse
Niemantsverdriet M, Nagle P, Chiu RK et al. deltaNp73 enhances promoter activity of TGF-B induced genes. PLoS One. 2012 [PMID: 23236396]
Leonard M K, Kommagani R, Payal V et al. DeltaNp63alpha regulates keratinocyte proliferation by controlling PTEN expression and localization. Cell Death Differ. 2011 Dec [PMID: 21637289]
Rosenbluth JM, Johnson K, Tang L et al. Evaluation of p63 and p73 antibodies for cross-reactivity. Cell Cycle. 2009 Nov 15 [PMID: 19855172]
Lin Yu-Li, Sengupta Shomit, Gurdziel Katherine et al. p63 and p73 transcriptionally regulate genes involved in DNA repair. PLoS Genet. 2009 Oct [PMID: 19816568] (IHC, Mouse) IHC Mouse
Show All 29 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for p73 Antibody (NB100-56674) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56674AF405
Alexa Fluor 488 Labeled NB100-56674AF488
Alexa Fluor 647 Labeled NB100-56674AF647
Alexa Fluor 700 Labeled NB100-56674AF700
Biotin Labeled NB100-56674B
DyLight 350 Labeled NB100-56674UV
DyLight 405 Labeled NB100-56674V
DyLight 488 Labeled NB100-56674G
DyLight 550 Labeled NB100-56674R
DyLight 650 Labeled NB100-56674C
DyLight 680 Labeled NB100-56674FR
DyLight 755 Labeled NB100-56674IR
HRP Labeled NB100-56674H

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Blogs on p73.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

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Bioinformatics

Gene Symbol TP73
Entrez
Uniprot