p70 S6 Kinase/S6K [p Ser411] Antibody - BSA Free

Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using NB100-82120 (left) or the same antibody preincubated with blocking peptide(right).

Western Blot: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Analysis of extracts from MCF cells untreated or treated with EGF using NB100-82120

Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Staining of methanol-fixed MCF7 cells using NB100-82120.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

p70 S6 Kinase/S6K [p Ser411] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit p70 S6 Kinase/S6K [p Ser411] Antibody - BSA Free (NB100-82120) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-p70 S6 Kinase/S6K Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase/S6K around the phosphorylation site of serine 411 (I-R-SP-P-R).
• Modification: p Ser411
• Specificity: Detects endogenous levels of p70 S6 Kinase/S6K only when phosphorylated at serine 411.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS6KB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  p70 S6 Kinase/S6K Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for p70 S6 Kinase/S6K [p Ser411] Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• p70 ribosomal S6 kinase alpha
• p70 S6 kinase alpha
• p70 S6 Kinase
• p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
• p70 S6 kinase, alpha 2
• p70 S6KA
• p70 S6K-alpha
• p70(S6K)-alpha
• p70-alpha
• p70-S6K
• P70S6K1
• PS6K
• ribosomal protein S6 kinase beta-1
• Ribosomal protein S6 kinase I
• ribosomal protein S6 kinase, 70kD, polypeptide 1
• ribosomal protein S6 kinase, 70kDa, polypeptide 1
• RPS6KB1
• S6K
• S6K1
• S6K1p70-S6K 1
• S6K-beta-1
• serine/threonine kinase 14 alpha
• Serine/threonine-protein kinase 14A
• STK14A

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p70 S6 Kinase/S6K Antibody (NB100-82120)(1)

Publication using NB100-82120

• Narayanan D, Tahseen D, Bartley BR et al. Trichodysplasia spinulosa polyomavirus small T antigen synergistically modulates S6 protein translation and DNA damage response pathways to shape host cell environment Virus genes 2022-01-09 [PMID: 35000075]

Bioinformatics

• Gene Symbol: RPS6KB1
• Entrez:
  • 6198(Human)
  • 72508(Mouse)
  • 83840(Rat)
• Uniprot:
  • P23443(Human)