Western blot analysis (0.5-4 ug/ml), immunoprecipitation (5-10 ug/ml), and Immunohistochemistry (20 ug/ml). However, the optimal conditions should be determined individually. The Phospho-p53 (Ser15) antibody detects ~53 kDa Ser15 phosphorylated p53. It does not react with non-phosphorylated p53 or p53 phosphorylated at other sites.
53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
Immunogen affinity purified
Alternate Names for p53 [p Ser15] Antibody
p53 tumor suppressor
P53cellular tumor antigen p53
transformation-related protein 53
tumor protein p53
Tumor suppressor p53
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNA-PK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for p53 Antibody (NB100-1913). (Showing 1 - 1 of 1 FAQs).
This product is powder or liquid? Could you suggest me the concentration for human sample (IHC-P)?
I am showing that this product comes in liquid form at 0.5 mg/mL and the suggested concentration for IHC is 20 ug/mL. This of course is just a guide and must be optimized by you based on how highly expressed the protein is in your samples.
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