p53 [p Ser15] Antibody

Images

 
Western Blot: p53 [p Ser15] Antibody [NB100-1913] - Western blot analysis of extract from UV treated (lane 2) and untreated (Lane 1) HeLa cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

p53 [p Ser15] Antibody Summary

Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser15 of human p53.
Modification
p Ser15
Specificity
The phospho-p53 (Ser15) detects ~53 kDa phosphorylated p53 at Ser15. It does not react with non-phosphorylated p53 or p53 phosphorylated at other sites.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TP53
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Western blot analysis (0.5-4 ug/ml), immunoprecipitation (5-10 ug/ml), and Immunohistochemistry (20 ug/ml). However, the optimal conditions should be determined individually. The Phospho-p53 (Ser15) antibody detects ~53 kDa Ser15 phosphorylated p53. It does not react with non-phosphorylated p53 or p53 phosphorylated at other sites.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
p53 Lysate (NBL1-17203)
Publications
Read Publications using
NB100-1913 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p53 [p Ser15] Antibody

  • Antigen NY-CO-13
  • BCC7
  • FLJ92943
  • LFS1
  • LFS1TRP53
  • p53 tumor suppressor
  • p53
  • P53cellular tumor antigen p53
  • Phosphoprotein p53
  • TP53
  • transformation-related protein 53
  • TRP53
  • tumor protein p53
  • Tumor suppressor p53

Background

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNA-PK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p53 Antibody (NB100-1913)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for p53 Antibody (NB100-1913). (Showing 1 - 1 of 1 FAQs).

  1. This product is powder or liquid? Could you suggest me the concentration for human sample (IHC-P)?
    • I am showing that this product comes in liquid form at 0.5 mg/mL and the suggested concentration for IHC is 20 ug/mL. This of course is just a guide and must be optimized by you based on how highly expressed the protein is in your samples.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on p53. Showing 1-10 of 16 blog posts - Show all blog posts.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

PRMT6: One Function, Many Roles
Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells. It regulates RNA processing, trafficking and nascent pre-RNA metabolism, receptor-mediated signal transduction, and transcriptional activation processes. P...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

p14 ARF is an Important Tumor Suppressor
The p14ARF (Alternative Reading Frame) tumor suppressor is a protein product of the alternative reading frame (ARF) of the human INK4a locus which regulates a series of cell cycle regulatory proteins to promote cell cycle arrest in response to abnorma...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol TP53
Entrez
Uniprot