p38 gamma/SAPK3 Antibody (212464) [Unconjugated]

Western blot shows lysates of C2C12 mouse myoblast cell line myoblast cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human p38 beta , p38 gamma, p38d and p38a (2 ng/lane) were included. A specific band for native p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

p38 gamma was detected in immersion fixed MCF-7 human breast cancer cell line (positive control, left panel) and MOLT-4 human acute lymphoblastic leukemia cell line (negative control, right panel) using Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in MCF-7 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.5 mg/mL. A specific band was detected for p38 gamma at approximately 46 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Muscle-specific deletion of the p38 alpha , p38 beta or p38 gamma gene does not affect endurance exercise-induced fiber-type transformation. Wild type, p38 alpha , p38 beta , and p38 gamma MKO mice were subjected to 4 weeks of voluntary running (Ex) or sedentary cage activity (Sed) followed by fiber-type and immunoblot analyses in plantaris muscles. A) PCR of genomic DNA with the appropriate primers (see Materials and Methods) for the loxP flanked p38 alleles in wild type (WT), heterozygous (+/−) and homozygous (−/−) mice with loxP-flanked p38 alleles. Only the homozygous mice with the Cre transgene (not shown) were considered p38 muscle-specific knockout (MKO) mice; B) Immunoblot analysis for p38 alpha and p38 gamma protein in plantaris muscles of p38 MKO mice in comparison with the wild type littermates. alpha -tubulin was used as a loading control; and C) Images of immunofluorescence staining of plantaris muscle sections with antibodies against myosin heavy chain IIb (Green), IIa (Blue) and I (Red). Appreciable increases in the percentage of type IIa fibers with concurrent decreases in type IIb fibers are noted in Ex group compared with Sed group among all four genetic backgrounds. The quantitative data is presented in Table 1. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19936205), licensed under a CC-BY license. Not internally tested by R&D Systems.

Muscle-specific deletion of the p38 alpha , p38 beta or p38 gamma gene does not affect endurance exercise-induced fiber-type transformation. Wild type, p38 alpha , p38 beta , and p38 gamma MKO mice were subjected to 4 weeks of voluntary running (Ex) or sedentary cage activity (Sed) followed by fiber-type and immunoblot analyses in plantaris muscles. A) PCR of genomic DNA with the appropriate primers (see Materials and Methods) for the loxP flanked p38 alleles in wild type (WT), heterozygous (+/−) and homozygous (−/−) mice with loxP-flanked p38 alleles. Only the homozygous mice with the Cre transgene (not shown) were considered p38 muscle-specific knockout (MKO) mice; B) Immunoblot analysis for p38 alpha and p38 gamma protein in plantaris muscles of p38 MKO mice in comparison with the wild type littermates. alpha -tubulin was used as a loading control; and C) Images of immunofluorescence staining of plantaris muscle sections with antibodies against myosin heavy chain IIb (Green), IIa (Blue) and I (Red). Appreciable increases in the percentage of type IIa fibers with concurrent decreases in type IIb fibers are noted in Ex group compared with Sed group among all four genetic backgrounds. The quantitative data is presented in Table 1. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19936205), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF
• Clone: 212464
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

p38 gamma/SAPK3 Antibody (212464) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human p38 gamma
  Ala7-Leu367
  Accession # P53778
• Specificity: Detects human, mouse, and rat p38 gamma in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant p38 alpha , p38 beta , or p38δ is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK12
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p38 gamma/SAPK3 Antibody (212464) [Unconjugated]

• EC 2.7.11
• ERK3
• ERK-6
• ERK6MAPK 12
• Extracellular signal-regulated kinase 6
• MAP kinase 12
• MAP kinase p38 gamma
• MAPK12
• mitogen-activated protein kinase 12
• mitogen-activated protein kinase 3
• Mitogen-activated protein kinase p38 gamma
• p38 gamma
• p38gamma
• PRKM12
• SAPK-3
• SAPK3EC 2.7.11.24
• Stress-activated protein kinase 3

Background

p38 mitogen-activated protein kinase (MAPK) gamma is a serine-threonine protein kinase that is also known as p38 gamma , MAPK12, stress-activated protein kinase-3 (SAPK3) and extracellular signal-regulated kinase-6 (ERK6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p38 gamma/SAPK3 Antibody (MAB1347)(5)

Publications using MAB1347

• Gilad, N;Mohanam, MP;Darlyuk-Saadon, I;Heng, CKM;Plaschkes, I;Benyamini, H;Berezhnoy, NV;Engelberg, D; Asynchronous Pattern of MAPKs' Activity during Aging of Different Tissues and of Distinct Types of Skeletal Muscle International journal of molecular sciences 2024-01-30 [PMID: 38338990] (Western Blot, Mouse)
• p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts. Biochem Biophys Res Commun, 2012-12-01;430(1):419-24. 12/1/2012 [PMID: 23206705]
• Stina K. Carlsson, J. Peter Gierow p38 Mitogen-activated protein kinase modulates exocrine secretion in rabbit lacrimal gland Cellular and Molecular Biology Letters 1/1/2012 [PMID: 22069054]
• Zafar Rasheed, Nahid Akhtar, Tariq M Haqqi Pomegranate extract inhibits the interleukin-1 beta -induced activation of MKK-3, p38 alpha -MAPK and transcription factor RUNX-2 in human osteoarthritis chondrocytes Arthritis Research & Therapy 10/18/2010 [PMID: 20955562]
• Korb A, Tohidast-Akrad M, Cetin E, Axmann R, Smolen J, Schett G Differential tissue expression and activation of p38 MAPK alpha, beta, gamma, and delta isoforms in rheumatoid arthritis. Arthritis Rheum., 2006-09-01;54(9):2745-56. 2006-09-01 [PMID: 16947383] (IHC, Immunoprecipitation, Western Blot, Human)

Review for p38 gamma/SAPK3 Antibody (MAB1347) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/04/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23206705
• Species: Human

Bioinformatics

• Gene Symbol: MAPK12
• Uniprot:
  • P53778()