p38 alpha Antibody (M138)

Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M138) [NBP3-23233] - Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38 alpha MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western Blot: p38 alpha Antibody (M138) [NBP3-23233] - Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38 alpha (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: M138
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

p38 alpha Antibody (M138) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone M138 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of rat p38a. This sequence is identical in human and mouse p38a. [UniProt# P70618]
• Specificity: This antibody detects a 38 kDa protein corresponding to the apparent molecular mass of p38 alpha on SDS-PAGE immunoblots of human Jurkat, A431, and HeLa cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK14
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 1:1000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for p38 alpha Antibody (M138)

• CSBP;CSBP1;CSBP2;CSPB1;EXIP;MAPK14;Mitogen-activated protein kinase;Mitogen-activated protein kinase 14;Mxi2;p38;p38ALPHA;PRKM14;PRKM15;RK;SAPK2A
• MAPK14
• p38 alpha
• SAPK2a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK14