p38 alpha Antibody (9F12) - BSA Free

Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry: p38 alpha Antibody (9F12) [NB110-96875] - Immunohistochemistry analysis using Mouse Anti-p38 alpha Monoclonal Antibody, Clone 9F12 (NB110-96875). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 alpha Monoclonal Antibody (NB110-96875) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Func, IHC, IP, MA
• Clone: 9F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

p38 alpha Antibody (9F12) - BSA Free Summary

• Immunogen: Full length recombinant protein expressed in E.coli cells
• Localization: Cytoplasm , Nucleus
• Specificity: Detects approx 38kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK14
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation
  • Microarray
  • Proximity Ligation Assay
  • Western Blot 1:1000
• Application Notes: Detects approx 38kDa protein corresponding to p38a MAPK when loaded with 6 ng of purified p38a by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. Use in Proximity ligation assay reported in scientific literature (PMID: 30987646).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for p38 alpha Antibody (9F12) - BSA Free

• CSBP;CSBP1;CSBP2;CSPB1;EXIP;MAPK14;Mitogen-activated protein kinase;Mitogen-activated protein kinase 14;Mxi2;p38;p38ALPHA;PRKM14;PRKM15;RK;SAPK2A
• MAPK14
• p38 alpha
• SAPK2a

Background

The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2alpha translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p38 alpha Antibody (NB110-96875)(2)

Publications using NB110-96875

• SchnOder L, Gasparoni G, NordstrOm K et al. Neuronal deficiency of p38 alpha-MAPK ameliorates symptoms and pathology of APP or Tau-transgenic Alzheimer's mouse models FASEB J. 2020-05-31 [PMID: 32475008] (Mouse)
• Koch, SR;Choi, H;Mace, EH;Stark, RJ; Toll-like receptor 3-mediated inflammation by p38 is enhanced by endothelial nitric oxide synthase knockdown Cell Commun. Signal 2019-04-15 [PMID: 30987646] (PLA, Human)

Bioinformatics

• Gene Symbol: MAPK14
• Entrez:
  • 1432(Human)
  • 81649(Rat)
• Uniprot:
  • Q16539()
  • NP_001306.1(Human)