P2Y12/P2RY12 Antibody (PSH04-48)

Immunohistochemistry-Frozen: P2Y12/P2RY12 Antibody (PSH04-48) [NBP3-32622] - Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-P2Y12/P2RY12 antibody (NBP3-32622) at 1/200 dilution.The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32622, green) at 1/200 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: P2Y12/P2RY12 Antibody (PSH04-48) [NBP3-32622] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-P2Y12/P2RY12 antibody (NBP3-32622) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32622) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

P2Y12/P2RY12 Antibody (PSH04-48) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide. (Uniprot: Q9H244)
• Localization: Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: P2RY12
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:1000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for P2Y12/P2RY12 Antibody (PSH04-48)

• ADPG-R
• Gi-coupled ADP receptor HORK3
• HORK3
• HORK3P2Y12 platelet ADP receptor
• P2RY12
• P2T(AC)
• P2Y purinoceptor 12
• P2Y(AC)
• P2Y(ADP)
• P2Y(cyc)
• P2Y12
• P2Y12ADP-glucose receptor
• purinergic receptor P2RY12
• purinergic receptor P2Y, G-protein coupled, 12
• putative G-protein coupled receptor
• SP1999
• SP1999G-protein coupled receptor SP1999

Background

P2Y12 is a Purinergic Receptor essential for the aggregation of blood platelets. There is evidence that mutations in P2Y12 cause a bleeding disorder, suggesting that knowledge of the P2Y12 receptor could advance the development of antiplatelet agents to treat cardiovascular diseases. Expression of P2Y12 has been reported in platelets, spinal cord, and many brain regions. ESTs for P2Y12 have been isolated from B-cell/lung/testis, brain, embryo, prostate, eye, kidney carcinoma, and colon carcinoma libraries. Cognate

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: P2RY12