p114RhoGEF Antibody

Western Blot: p114RhoGEF Antibody [NBP1-32816] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ARHGEF18 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: p114RhoGEF Antibody [NBP1-32816] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: p114RhoGEF Antibody [NBP1-32816] - Rat spleen. ARHGEF18 stained by ARHGEF18 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: p114RhoGEF Antibody [NBP1-32816] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ARHGEF18 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: p114RhoGEF Antibody [NBP1-32816] - Mouse thymus gland. ARHGEF18 stained by ARHGEF18 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with p114RhoGEF antibody (NBP1-32816) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

p114RhoGEF Antibody Summary

• Description: Novus Biologicals Rabbit p114RhoGEF Antibody (NBP1-32816) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human p114RhoGEF. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARHGEF18
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 131 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for p114RhoGEF Antibody

• 114 kDa Rho-specific guanine nucleotide exchange factor
• EC 3.4.24
• EC 3.6.1
• KIAA0521rho guanine nucleotide exchange factor 18
• MGC15913
• p114RhoGEF
• P114-RhoGEF
• P114-RHO-GEF
• Rho/Rac guanine nucleotide exchange factor (GEF) 18
• Rho/Rac guanine nucleotide exchange factor 18
• Rho-specific guanine nucleotide exchange factor p114
• SA-RhoGEF
• Septin-associated RhoGEF

Background

Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ARHGEF18