p114RhoGEF Antibody

Images

 
Western Blot: p114RhoGEF Antibody [NBP1-32816] - Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: p114RhoGEF Antibody [NBP1-32816] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.43 mg/ml

Order Details

p114RhoGEF Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ARHGEF18. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARHGEF18
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (88%), Rat (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.43 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p114RhoGEF Antibody

  • 114 kDa Rho-specific guanine nucleotide exchange factor
  • EC 3.4.24
  • EC 3.6.1
  • KIAA0521rho guanine nucleotide exchange factor 18
  • MGC15913
  • p114RhoGEF
  • P114-RhoGEF
  • P114-RHO-GEF
  • Rho/Rac guanine nucleotide exchange factor (GEF) 18
  • Rho/Rac guanine nucleotide exchange factor 18
  • Rho-specific guanine nucleotide exchange factor p114
  • SA-RhoGEF
  • Septin-associated RhoGEF

Background

Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ARHGEF18