OX40 Ligand/TNFSF4 Antibody (182601) [Unconjugated]

In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050) and sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484), Recombinant Mouse OX40 Ligand/TNFSF4 (Catalog # 1236-OX) stimulates IL‑2 secretion in mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IL‑2 Quantikine ELISA Kit (Catalog # M2000). Under these conditions, IL‑2 secretion elicited by Recombinant Mouse OX40 Ligand/TNFSF4 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse OX40 Ligand/TNFSF4 Monoclonal Antibody (Catalog # MAB1236). The ND50 is typically 0.01-0.05 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clone: 182601
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

OX40 Ligand/TNFSF4 Antibody (182601) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse OX40 Ligand/TNFSF4
  Gln49-Leu198
  Accession # P43488
• Specificity: Detects mouse OX40 Ligand/TNFSF4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) APRIL, rhGITR Ligand, rhLIGHT, rhLT alpha 1/ beta 2, rhLT alpha 2/ beta 1, recombinant cotton rat TNF‑ alpha , recombinant mouse (rm) TNF‑ alpha , rmTRAIL, rhTRANCE, rmTRANCE, rmTWEAK, or rhVEGI is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFSF4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.01-0.05 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OX40 Ligand/TNFSF4 Antibody (182601) [Unconjugated]

• CD134 ligand
• CD134L
• CD252 antigen
• CD252
• Glycoprotein Gp34
• gp34
• OX40 antigen ligand
• OX40 Ligand
• OX40L
• OX-40L
• OX4OL
• TAX transcriptionally-activated glycoprotein 1
• tax-transcriptionally activated glycoprotein 1 (34kD)
• tax-transcriptionally activated glycoprotein 1, 34kD
• TNFSF4
• tumor necrosis factor (ligand) superfamily, member 4
• tumor necrosis factor ligand superfamily member 4
• TXGP1

Background

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Murine OX40L cDNA encodes a 198 amino acid (aa) protein comprised of a 28 aa N-terminal cytoplasmic domain, a 20 aa transmembrane segment, and a 150 aa C-terminal extracellular domain (1). Human and mouse OX40L share 46% sequence identity at the amino acid level (1). OX40L is expressed on activated antigen presenting cells such as B cells, macrophages, dendritic cells, and on endothelial cells at the site of inflammation. The receptor for OX40L is OX40 (CD134) which is expressed predominantly on activated CD4⁺ T cells. Expression of OX40 is transient following engagement of T cell receptors (2). Ligation of OX40L by OX40 stimulates proliferation and differentiation of activated B cells, and increases immunoglobulin secretion (3, 4). The expression of OX40L on B cells is upregulated by CD40 ligation (3). Engagement of the OX40‑OX40L system has co-stimulatory effects on T cells by stimulating the production of cytokines by T helper cells and increasing the survival of memory T cells (2, 5). Blocking of the OX40‑OX40L interaction in vitro inhibits co-stimulation resulting in decreased T cell proliferation and adhesion of T cells to endothelial cells. Inhibition of the OX40‑OX40L interaction in disease models has beneficial effects in acute graft-versus-host disease, inflammatory bowel disease and decreases the development of collagen-induced arthritis and experimental leishmaniasis (6).

1. Baum, P.R. et al. (1994) EMBO J. 13:3992.
2. Gramaglia, I. et al. (1999) J. Immunol. 161:6510.
3. Stuber, E. et al. (1995) Immunity 2:507.
4. Malstrom, V. et al. (2001) J. Immunol. 166:6972.
5. Maxwell, J.R. et al. (2000) J. Immunol. 164:107.
6. Weinberg, A.D. (2002) Trends Immunol. 23:102.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OX40 Ligand/TNFSF4 Antibody (MAB1236)(5)

Publications using MAB1236

• Hua Jin, Chunpan Zhang, Chengyang Sun, Xinyan Zhao, Dan Tian, Wen Shi et al. OX40 expression in neutrophils promotes hepatic ischemia/reperfusion injury JCI Insight 11/1/2019 [PMID: 31672934]
• L Sun, C Dai, J Wu, C Chen, X Wu TSLP-activated dendritic cells induce T helper type 2 inflammation in Aspergillus fumigatus keratitis Exp. Eye Res., 2018-03-14;0(0):. 2018-03-14 [PMID: 29550278] (Western Blot, Mouse)
• Yongyan Chen, Rui Sun, Xunyao Wu, Min Cheng, Haiming Wei, Zhigang Tian CD4+CD25+ Regulatory T Cells Inhibit Natural Killer Cell Hepatocytotoxicity of Hepatitis B Virus Transgenic Mice via Membrane-Bound TGF-beta and OX40 Journal of Innate Immunity 1/1/2016 [PMID: 26067079]
• Yokota-Nakatsuma A, Takeuchi H, Ohoka Y, Kato C, Song S, Hoshino T, Yagita H, Ohteki T, Iwata M Retinoic acid prevents mesenteric lymph node dendritic cells from inducing IL-13-producing inflammatory Th2 cells. Mucosal Immunol, 2013-11-13;7(4):786-801. 2013-11-13 [PMID: 24220301] (Neutralization, Mouse)
• Peters M, Dudziak K, Stiehm M T-cell polarization depends on concentration of the danger signal used to activate dendritic cells. Immunol. Cell Biol., 2010-02-02;88(5):537-44. 2010-02-02 [PMID: 20125117] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: TNFSF4
• Uniprot:
  • P43488()