OVCA1 Antibody (2C5) - Azide and BSA Free

Western Blot: OVCA1 Antibody (2C5) [H00001801-M02] - DPH1 monoclonal antibody (M02), clone 2C5 Analysis of DPH1 expression in HeLa.

Immunocytochemistry/Immunofluorescence: OVCA1 Antibody (2C5) [H00001801-M02] - Analysis of monoclonal antibody to DPH1 on HeLa cell. Antibody concentration 10 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

OVCA1 Antibody (2C5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DPH1 (NP_001374, 216 a.a. ~ 324 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNVPAYRYDPYSKVLSREHYDHQRMQAARQEAIATARSAKSWGLILGTLGRQGSPKILEHLESRLRALGLSFVRLL
• Specificity: DPH1 (2C5)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DPH1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate for WB. It has been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for OVCA1 Antibody (2C5) - Azide and BSA Free

• Diphthamide biosynthesis protein 2 homolog-like 1
• Diphthamide biosynthesis protein 2-like
• diptheria toxin resistance protein required for diphthamide biosynthesis(Saccharomyces)-like 1
• diptheria toxin resistance protein required for diphthamide biosynthesis-like 1
• diptheria toxin resistance protein required for diphthamide biosynthesis-like 1(S. cerevisiae)
• DPH1 homolog (S. cerevisiae)
• DPH1 homolog
• DPH2L
• DPH2L1diphthamide biosynthesis protein 1
• DPH2-like 1
• DPH-like 1 (S. cerevisiae)
• DPH-like 1
• FLJ33211
• hsDph1
• Ovarian cancer-associated gene 1 protein
• ovarian tumor suppressor candidate 1
• OVCA1candidate tumor suppressor in ovarian cancer 1

Background

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPH1
• Entrez:
  • 1801(Human)
• OMIM:
  • 603527(Human)
• Uniprot:
  • NP_001374(Human)