Otubain-1 Antibody (OTI5B8)

Western Blot: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Otubain-1.

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Adenocarcinoma of Human colon tissue.

Flow Cytometry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by OTUB1 antibody.

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Human Kidney tissue.

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Carcinoma of Human prostate tissue.

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Human prostate tissue.

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Adenocarcinoma of Human endometrium tissue.

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Human tonsil tissue within the normal limits using OTUB1 mouse monoclonal antibody.

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Carcinoma of Human kidney tissue.

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Human pancreas tissue .

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Human liver tissue.

• Reactivity: Hu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI5B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Otubain-1 Antibody (OTI5B8) Summary

• Description: Novus Biologicals Mouse Otubain-1 Antibody (OTI5B8) (NBP2-45879) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human OTUB1(NP_060140) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: OTUB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 31.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Otubain-1 Antibody (OTI5B8)

• Deubiquitinating enzyme OTUB1
• EC 3.1.2
• EC 3.4.19.12
• FLJ20113
• FLJ40710
• hOTU1
• MGC111158
• OTB1
• OTB1MGC4584
• OTU domain, ubiquitin aldehyde binding 1
• OTU domain-containing ubiquitin aldehyde-binding protein 1
• OTU1
• OTU1OTU-domain Ubal-binding 1
• OTUB1
• Otubain1
• Otubain-1
• ubiquitin thioesterase OTUB1
• ubiquitin-specific protease otubain 1
• Ubiquitin-specific-processing protease OTUB1

Background

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTUB1