Otubain-1 Antibody (GT611) - Azide and BSA Free

Western Blot: Otubain-1 Antibody (GT611) [NBP3-13627] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Otubain-1 antibody [GT611] (NBP3-13627) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Otubain-1 Antibody (GT611) [NBP3-13627] - Otubain-1 antibody [GT611] detects Otubain-1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Otubain-1 stained by Otubain-1 antibody [GT611] (NBP3-13627) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.

• Reactivity: Mu, Rt, Hu, Po, Bv, Ca
• Applications: WB, ICC/IF
• Clone: GT611
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Otubain-1 Antibody (GT611) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of mouse Otubain-1. The exact sequence is proprietary.
• Predicted Species: Human (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: OTUB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Otubain-1 Antibody (GT611) - Azide and BSA Free

• Deubiquitinating enzyme OTUB1
• EC 3.1.2
• EC 3.4.19.12
• FLJ20113
• FLJ40710
• hOTU1
• MGC111158
• OTB1
• OTB1MGC4584
• OTU domain, ubiquitin aldehyde binding 1
• OTU domain-containing ubiquitin aldehyde-binding protein 1
• OTU1
• OTU1OTU-domain Ubal-binding 1
• OTUB1
• Otubain1
• Otubain-1
• ubiquitin thioesterase OTUB1
• ubiquitin-specific protease otubain 1
• Ubiquitin-specific-processing protease OTUB1

Background

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTUB1