OTP Antibody (CL11225)

Immunocytochemistry/Immunofluorescence: OTP Antibody (CL11222) [NBP3-15136] - Staining of MCF7 cells using the Anti-OTP monoclonal antibody, showing specific staining in nuclear bodies and in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively.

Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human hypothalamus shows moderate to strong nuclear positivity in a subset of neurons.

Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human lung tumor (typical carcinoid) shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of rat hypothalamus shows strong nuclear positivity in a subset of neurons.

Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human lymph node shows no positivity in lymphoid cells as expected.

• Reactivity: Hu, Rt, Mu
• Applications: ICC/IF, IHC, IHC-P
• Clone: CL11225
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

OTP Antibody (CL11225) Summary

• Immunogen: This antibody was developed against a recombinant protein. Actual immunogen sequence is proprietary information.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: OTP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for OTP Antibody (CL11225)

• homeobox protein orthopedia
• MGC3161
• orthopedia homeobox
• orthopedia homolog (Drosophila)
• orthopedia homolog

Background

OTP encodes a member of the homeodomain (HD) family. HD family proteins are helix-turn-helix transcription factors that play key roles in the specification of cell fates. This protein may function during brain development. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTP