Osteocrin Antibody (311417) [Unconjugated]

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clone: 311417
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Osteocrin Antibody (311417) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Osteocrin
  Val28-Gly130
  Accession # P61364
• Specificity: Detects human and mouse Osteocrin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: OSTN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteocrin Antibody (311417) [Unconjugated]

• Musclin
• Osteocrin
• OSTN

Background

Mouse Osteocrin, also known as Musclin, is a secreted protein that is primarily expressed in bone and muscle. It is synthesized as an 11 kDa proprotein that undergoes proteolytic processing to generate a mature 50 amino acid (5 kDa) C-terminal active peptide. It was found to modulate osteoblast differentation and to regulate glucose metabolism in muscles. Mouse Osteocrin proprotein shares 77% and 78% amino acid sequence identity with the rat and human protein, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Osteocrin Antibody (MAB2620)(1)

Publication using MAB2620

• H Watanabe-T, H Ochi, A Chiba, A Matsuo, Y Kanai, S Fukuhara, N Ito, K Sako, T Miyazaki, K Tainaka, I Harada, S Sato, Y Sawada, N Minamino, S Takeda, HR Ueda, A Yasoda, N Mochizuki Mechanical load regulates bone growth via periosteal Osteocrin Cell Reports, 2021-07-13;36(2):109380. 2021-07-13 [PMID: 34260913] (ELISA Capture, Mouse)

Bioinformatics

• Gene Symbol: OSTN
• Uniprot:
  • P61364()