Rat Osteocalcin ELISA Kit (Colorimetric)

ELISA: Rat Osteocalcin ELISA Kit (Colorimetric) [NBP2-68153] - Samples were spiked with high concentrations of Rat Osteocalcin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Osteocalcin ELISA Kit (Colorimetric) [NBP2-68153] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.78-50 ng/mL
• Sensitivity: 0.47 ng/mL

Rat Osteocalcin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat OC/BGP in samples. No significant cross-reactivity or interference between Rat OC/BGP and analogues was observed.
• Standard Curve Range: 0.78-50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.33%
• Intra-Assay: CV% < 5.02%
• Spike Recovery: 85-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: BGLAP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Osteocalcin ELISA Kit (Colorimetric)

• BGLAP
• BGP
• bone gamma-carboxyglutamate (gla) protein (osteocalcin)
• bone gamma-carboxyglutamate (gla) protein
• Bone Gla protein
• Gamma-carboxyglutamic acid-containing protein
• OC
• OCN
• Osteocalcin

Background

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Osteocalcin ELISA Kit (NBP2-68153)(8)

Publications using NBP2-68153

• Huang Y, Dou Y, Yang B et al. Nicotinamide mononucleotide supplementation mitigates osteopenia induced by modeled microgravity in rats Cell stress & chaperones 2023-05-17 [PMID: 37195399] (ELISA)
• Ishikawa M, Kanzaki H, Kodera R et al. Early diagnosis of aortic calcification through dental X-ray examination for dental pulp stones Research Square 2023-08-16 (ELISA)
• Ahn JW, Kim HS, Damodar K et al. Styphnolobium japonicum Fruit and Germinated Soybean Embryo Complex Extract for Postmenopausal-Symptom Relief. Nutrients 2024-10-16 [PMID: 39408264] (ELISA)
• Elseweidy MM, Ali AE, Hassanin SM, Mahmoud YK Empagliflozin ameliorates liver fibrosis in NASH rat model via targeting hepatic NF-?B/SOX9/OPN signaling and osteocalcin level Naunyn-Schmiedeberg's archives of pharmacology 2023-11-14 [PMID: 37962587] (ELISA, Rat)
• Stacy Schkoda, Brian Horman, Shannah K. Witchey, Anton Jansson, Soraia Macari, Heather B. Patisaul Skeletal effects following developmental flame-retardant exposure are specific to sex and chemical class in the adult Wistar rat Frontiers in Toxicology 2023-07-27 [PMID: 37577032] (ELISA, Rat)
• Kim M, Kim HS, Oh J et Al. Ameliorative effects of Kyung-Ok-Ko and its mixture with Pueraria lobata Ohwi on postmenopausal osteoporosis by promoting phytoestrogenic activity in rats Front Nutr 2023-06-26 [PMID: 37435569] (ELISA, Rat)
• Zhang Z, Yue L, Wang Y et al. A circRNA-miRNA-mRNA network plays a role in the protective effect of diosgenin on alveolar bone loss in ovariectomized rats BMC Complement Med Ther 2020-07-14 [PMID: 32664914]
• Malek-Khatabi A, Javar Ha, Dashtimoghadam E et Al. In situ bone tissue engineering using gene delivery nanocomplexes Acta Biomater 2020-03-08 [PMID: 32160962] (ELISA, Rat)

FAQs for Osteocalcin ELISA Kit (NBP2-68153). (Showing 1 - 2 of 2 FAQs).

1. I am interested in your Rat Osteocalcin ELISA Kit (Colorimetric), Cat#NBP2-68153. Could you please tell us whether there are the components containing recombinant protein in this kit? If yes, could you tell us the expression systems of the recombinant protein?For Example,  E.coli, Yeast, HEK293 cells, CHO cells and insect cells etc?
   • The reference standard is a recombinant Rat mature Osteocalcin (50~99aa) proitein which was experimentally expressed using E.coli host.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BGLAP