Mouse Osteocalcin ELISA Kit (Colorimetric)

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ELISA: Mouse Osteocalcin ELISA Kit (Colorimetric) [NBP2-68151] - Samples were spiked with high concentrations of Mouse Osteocalcin and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Mouse Osteocalcin ELISA Kit (Colorimetric) [NBP2-68151] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.39 - 25 ng/mL
Sensitivity
0.23 ng/mL

Order Details

Mouse Osteocalcin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse OC/BGP in samples. No significant cross-reactivity or interference between Mouse OC/BGP and analogues was observed.
Standard Curve Range
0.39 - 25 ng/mL
Sensitivity
0.23 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.37%
Intra-Assay
CV% < 5.57%
Spike Recovery
88-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
BGLAP

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publications using
NBP2-68151 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse Osteocalcin ELISA Kit (Colorimetric)

  • BGLAP
  • BGP
  • bone gamma-carboxyglutamate (gla) protein (osteocalcin)
  • bone gamma-carboxyglutamate (gla) protein
  • Bone Gla protein
  • Gamma-carboxyglutamic acid-containing protein
  • OC
  • OCN
  • Osteocalcin

Background

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Osteocalcin ELISA Kit (NBP2-68151)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA.


Filter By Application
ELISA
(8)
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Filter By Species
Mouse
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-68151 Applications Species
Kubi J, Brah A, Cheung K et al. A new osteogenic protein isolated from Dioscorea opposita Thunb accelerates bone defect healing through the mTOR signaling axis Bioactive Materials 2023-09-01 [PMID: 37152710] (ELISA) ELISA
Bedree JK, Kerns K, Chen T et al. Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight Cell reports 2023-04-10 [PMID: 37080202] (ELISA) ELISA
Xiong J, Liu X, Zhang Z et al. R13, a TrkB Agonist Prodrug, Inhibits Asparagine Endopeptidase (AEP) and Increases Osteoprotegerin (OPG), Preventing Bone Loss Nat Commun 2022-08-16 [PMID: 35973996] (ELISA) ELISA
Lu K, Shi TS, Shen Sy et al. Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression Cell metabolism 2022-03-01 [PMID: 35235775]
Wang Y, Herroon M, Zielske S Et al. Use of FVB Myc-CaP cells as an immune competent, androgen receptor positive, mouse model of prostate cancer bone metastasis Journal of Bone Oncology 2021-10-01 [PMID: 34466376]
Lu Y, Wang Y, Zhang H et al. Solubilized Cartilage ECM Facilitates the Recruitment and Chondrogenesis of Endogenous BMSCs in Collagen Scaffolds for Enhancing Microfracture Treatment ACS applied materials & interfaces 2021-05-20 [PMID: 34014092] (ELISA) ELISA
Song C, Cao J, Lei Y et al. Nuciferine prevents bone loss by disrupting multinucleated osteoclast formation and promoting type H vessel formation FASEB J. 2020-02-10 [PMID: 32039519] (ELISA, Mouse) ELISA Mouse
Sun W, Chi S, Li Y et al. The mechanosensitive Piezo1 channel is required for bone formation Elife 2019-07-10 [PMID: 31290742] (ELISA, Mouse) ELISA Mouse
Goel PN, Moharrer Y, Hebb JH et al. Suppression of Notch Signaling in Osteoclasts Improves Bone Regeneration and Healing J. Orthop. Res. 2019-06-05 [PMID: 31166033]
Lu K, Shi T, Shen S et al. Lecithin-Cholesterol Acyltransferase Induced By PPSac alpha Deficiency in Liver Regulates the Homeostasis of Liver/Bone Axis and Ameliorates Hepatic Osteodystrophy Diseas SSRN Electronic Journal 2021-05-20 (ELISA) ELISA
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Osteocalcin ELISA Kit (NBP2-68151). (Showing 1 - 1 of 1 FAQs).

  1. Is mouse Osteocalcin ELISA Kit specific to mouse only? I am wondering if it could be used to quantify rat OCN correctly?
    • When tested in the lab with rat samples the cross-reactivity is about 22.63%. Thus we do not recommend the Mouse Osteocalcin ELISA Kit for rat samples.

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Bioinformatics

Gene Symbol BGLAP