Mouse Osteocalcin ELISA Kit (Colorimetric)

Mouse Osteocalcin ELISA Kit (Colorimetric) - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids.
• Standard Curve Range: 0.39 - 25 ng/mL
• Sensitivity: 0.23 ng/mL

Mouse Osteocalcin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse OC/BGP in samples. No significant cross-reactivity or interference between Mouse OC/BGP and analogues was observed.
• Standard Curve Range: 0.39 - 25 ng/mL
• Sensitivity: 0.23 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.37%
• Intra-Assay: CV% < 5.57%
• Spike Recovery: 88-105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: BGLAP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Osteocalcin ELISA Kit (Colorimetric)

• BGLAP
• BGP
• bone gamma-carboxyglutamate (gla) protein (osteocalcin)
• bone gamma-carboxyglutamate (gla) protein
• Bone Gla protein
• Gamma-carboxyglutamic acid-containing protein
• OC
• OCN
• Osteocalcin

Background

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Osteocalcin ELISA Kit (NBP2-68151)(9)

Publications using NBP2-68151

• Xiong J, Liu X, Zhang Z et al. R13, a TrkB Agonist Prodrug, Inhibits Asparagine Endopeptidase (AEP) and Increases Osteoprotegerin (OPG), Preventing Bone Loss Nat Commun Aug 16 2022 [PMID: 35973996] (ELISA)
• Lu K, Shi TS, Shen Sy et al. Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression Cell metabolism Mar 1 2022 [PMID: 35235775]
• Song C, Cao J, Lei Y et al. Nuciferine prevents bone loss by disrupting multinucleated osteoclast formation and promoting type H vessel formation FASEB J. Feb 10 2020 [PMID: 32039519] (ELISA, Mouse)
• Sun W, Chi S, Li Y et al. The mechanosensitive Piezo1 channel is required for bone formation Elife Jul 10 2019 [PMID: 31290742] (ELISA, Mouse)
• Goel PN, Moharrer Y, Hebb JH et al. Suppression of Notch Signaling in Osteoclasts Improves Bone Regeneration and Healing J. Orthop. Res. 2019 Jun 05 [PMID: 31166033]
• Lu K, Shi T, Shen S et al. Lecithin-Cholesterol Acyltransferase Induced By PPSac alpha Deficiency in Liver Regulates the Homeostasis of Liver/Bone Axis and Ameliorates Hepatic Osteodystrophy Diseas SSRN Electronic Journal May 20 2021 (ELISA)
• Wang Y, Herroon M, Zielske S Et al. Use of FVB Myc-CaP cells as an immune competent, androgen receptor positive, mouse model of prostate cancer bone metastasis Journal of Bone Oncology Oct 1 2021 [PMID: 34466376]
• Lu Y, Wang Y, Zhang H et al. Solubilized Cartilage ECM Facilitates the Recruitment and Chondrogenesis of Endogenous BMSCs in Collagen Scaffolds for Enhancing Microfracture Treatment ACS applied materials & interfaces May 20 2021 [PMID: 34014092] (ELISA)
• Li X, Wang L, Huang B et al. Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss Science Advances Nov 18 2020 [PMID: 33208358] (ELISA)

FAQs for Osteocalcin ELISA Kit (NBP2-68151). (Showing 1 - 1 of 1 FAQs).

1. Is mouse Osteocalcin ELISA Kit specific to mouse only? I am wondering if it could be used to quantify rat OCN correctly?
   • When tested in the lab with rat samples the cross-reactivity is about 22.63%. Thus we do not recommend the Mouse Osteocalcin ELISA Kit for rat samples.

Bioinformatics

• Gene Symbol: BGLAP