Mouse Osteocalcin ELISA Kit (Colorimetric)

ELISA: Mouse Osteocalcin ELISA Kit (Colorimetric) [NBP2-68151] - Samples were spiked with high concentrations of Mouse Osteocalcin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Osteocalcin ELISA Kit (Colorimetric) [NBP2-68151] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.39 - 25 ng/mL
• Sensitivity: 0.23 ng/mL

Mouse Osteocalcin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse OC/BGP in samples. No significant cross-reactivity or interference between Mouse OC/BGP and analogues was observed.
• Standard Curve Range: 0.39 - 25 ng/mL
• Sensitivity: 0.23 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.37%
• Intra-Assay: CV% < 5.57%
• Spike Recovery: 88-105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: BGLAP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Osteocalcin ELISA Kit (Colorimetric)

• BGLAP
• BGP
• bone gamma-carboxyglutamate (gla) protein (osteocalcin)
• bone gamma-carboxyglutamate (gla) protein
• Bone Gla protein
• Gamma-carboxyglutamic acid-containing protein
• OC
• OCN
• Osteocalcin

Background

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Osteocalcin ELISA Kit (NBP2-68151)(14)

Publications using NBP2-68151

• Tânia Amorim, Naveen Gv Kumar, Natalie L David, William Dion, Trishya Pagadala, Nandini K Doshi, Bokai Zhu, Andrey Parkhitko, Matthew L Steinhauser, Pouneh K Fazeli Methionine and one carbon metabolism as a regulator of bone remodeling with fasting. JCI insight 2024-05-21 [PMID: 38780544]
• Jaymes Fuller, Katherine Sares Lefferts, Pooja Shah, Jessica A. Cottrell, Alberto Falchetti, Jose Luis Pérez-Castrillón, Ricardo Usategui-Martín Methodology and Characterization of a 3D Bone Organoid Model Derived from Murine Cells International Journal of Molecular Sciences 2024-04-11 [PMID: 38673812]
• Kubi J, Brah A, Cheung K et al. A new osteogenic protein isolated from Dioscorea opposita Thunb accelerates bone defect healing through the mTOR signaling axis Bioactive Materials 2023-09-01 [PMID: 37152710] (ELISA)
• Bedree JK, Kerns K, Chen T et al. Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight Cell reports 2023-04-10 [PMID: 37080202] (ELISA)
• Belle Yu‐Hsuan Wang, Yi‐Fan Chen, Allen Wei‐Ting Hsiao, Wan‐Jing Chen, Chien‐Wei Lee, Oscar Kuang‐Sheng Lee Ginkgolide B facilitates muscle regeneration via rejuvenating osteocalcin‐mediated bone‐to‐muscle modulation in aged mice Journal of Cachexia, Sarcopenia and Muscle 2023-04-19 [PMID: 37076950]
• Xiong J, Liu X, Zhang Z et al. R13, a TrkB Agonist Prodrug, Inhibits Asparagine Endopeptidase (AEP) and Increases Osteoprotegerin (OPG), Preventing Bone Loss Nat Commun 2022-08-16 [PMID: 35973996] (ELISA)
• Lu K, Shi TS, Shen Sy et al. Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression Cell metabolism 2022-03-01 [PMID: 35235775]
• Wang Y, Herroon M, Zielske S Et al. Use of FVB Myc-CaP cells as an immune competent, androgen receptor positive, mouse model of prostate cancer bone metastasis Journal of Bone Oncology 2021-10-01 [PMID: 34466376]
• Lu Y, Wang Y, Zhang H et al. Solubilized Cartilage ECM Facilitates the Recruitment and Chondrogenesis of Endogenous BMSCs in Collagen Scaffolds for Enhancing Microfracture Treatment ACS applied materials & interfaces 2021-05-20 [PMID: 34014092] (ELISA)
• Song C, Cao J, Lei Y et al. Nuciferine prevents bone loss by disrupting multinucleated osteoclast formation and promoting type H vessel formation FASEB J. 2020-02-10 [PMID: 32039519] (ELISA, Mouse)
• Sun W, Chi S, Li Y et al. The mechanosensitive Piezo1 channel is required for bone formation Elife 2019-07-10 [PMID: 31290742] (ELISA, Mouse)
• Goel PN, Moharrer Y, Hebb JH et al. Suppression of Notch Signaling in Osteoclasts Improves Bone Regeneration and Healing J. Orthop. Res. 2019-06-05 [PMID: 31166033]
• Lu K, Shi T, Shen S et al. Lecithin-Cholesterol Acyltransferase Induced By PPSac alpha Deficiency in Liver Regulates the Homeostasis of Liver/Bone Axis and Ameliorates Hepatic Osteodystrophy Diseas SSRN Electronic Journal 2021-05-20 (ELISA)
• Li X, Wang L, Huang B et al. Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss Science Advances 2020-11-18 [PMID: 33208358] (ELISA)

FAQs for Osteocalcin ELISA Kit (NBP2-68151). (Showing 1 - 2 of 2 FAQs).

1. Is mouse Osteocalcin ELISA Kit specific to mouse only? I am wondering if it could be used to quantify rat OCN correctly?
   • When tested in the lab with rat samples the cross-reactivity is about 22.63%. Thus we do not recommend the Mouse Osteocalcin ELISA Kit for rat samples.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BGLAP