Osteoactivin/GPNMB Antibody [Biotin]

Intracerebroventricular treatment with 2-hydroxypropyl-beta -cyclodextrin (HP-beta -CD) attenuated the abnormal increase of glycoprotein nonmetastatic melanoma protein B (GPNMB) expression in Npc1−/− mice. Assays of GPNMB levels in brain using ELISA (A) and immunohistochemical staining of GPNMB (B) were performed. Scale bar: 100 μm. Data are presented as the mean ± S.E. (n = 4–7). *** p < 0.01 compared with the wild-type group. ### p < 0.001 compared with the saline group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33466390), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Osteoactivin/GPNMB Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Osteoactivin/GPNMB
  Lys23-Asn502
  Accession # NP_444340
• Specificity: Detects mouse Osteoactivin/GPNMB in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Osteoactivin, rhSyndecan-4, and recombinant mouse Syndecan-4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPNMB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Osteoactivin/GPNMB Antibody (Catalog # AF2330)
  Standard: Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteoactivin/GPNMB Antibody [Biotin]

• DC-HIL
• glycoprotein (transmembrane) nmb
• glycoprotein NMB
• GPNMB
• HGFIN
• HGFINglycoprotein nmb-like protein
• NMBosteoactivin
• Osteoactivin
• Transmembrane glycoprotein HGFIN
• transmembrane glycoprotein NMB

Background

Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.

1. Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
2. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
3. Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Osteoactivin/GPNMB Antibody (BAF2330)(4)

Publications using BAF2330

• Belgiovine, C;Digifico, E;Erreni, M;Putignano, AR;Mannarino, L;Valentino, S;Grizzi, F;Pasqualini, F;Recordati, C;Bertola, L;Zucali, P;Pistillo, D;Paleari, V;Mantovani, A;D'Incalci, M;Marchesi, F;Allavena, P; Malignant mesothelioma-associated inflammatory microenvironment promotes tumor progression via GPNMB Journal of translational medicine 2025-04-18 [PMID: 40251684] (Immunohistochemistry, Human)
• Androvic P, Schifferer M, Perez Anderson K et al. Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury Nature communications 7/11/2023 [PMID: 37433806]
• M Fukaura, Y Ishitsuka, S Shirakawa, N Ushihama, Y Yamada, Y Kondo, T Takeo, N Nakagata, K Motoyama, T Higashi, H Arima, Y Kurauchi, T Seki, H Katsuki, K Higaki, M Matsuo, T Irie Intracerebroventricular Treatment with 2-Hydroxypropyl-&amp;beta-Cyclodextrin Decreased Cerebellar and Hepatic Glycoprotein Nonmetastatic Melanoma Protein B (GPNMB) Expression in Niemann-Pick Disease Type C Model Mice International Journal of Molecular Sciences, 2021-01-05;22(1):. 2021-01-05 [PMID: 33466390] (IHC, Mouse)
• Alice S Kaanta, Carl Virtanen, Laura M Selfors, Joan S Brugge, Benjamin G Neel Evidence for a multipotent mammary progenitor with pregnancy-specific activity Breast Cancer Research 2013-08-15 [PMID: 23947835] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: GPNMB
• Uniprot:
  • NP_444340()