OSBPL5 Antibody

Western Blot: OSBPL5 Antibody [NB100-57071] - (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: OSBPL5 Antibody [NB100-57071] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: OSBPL5 Antibody [NB100-57071] - Staining of Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: OSBPL5 Antibody [NB100-57071] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: OSBPL5 Antibody [NB100-57071] - (0.3ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: OSBPL5 Antibody [NB100-57071] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: OSBPL5 Antibody [NB100-57071] - Staining of Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

OSBPL5 Antibody Summary

• Immunogen: Peptide with sequence KEEAFLRRRFSLC corresponding to N-Terminus according to NP_065947.1, NP_663613.1, NP_001137535.1.
• Epitope: KEEAFLRRRFSLC
• Specificity: This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1; NP_001137535.1) encode the same isoform.
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OSBPL5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.03 - 0.3 ug/ml
• Application Notes: Western blot: Approx. 100kDa band observed in lysates of cell line Jurkat and in Rat Spleen lysates (calculated MW of 98.4kDa according to Human NP_065947.1 and 101.7kDa according to Rat NP_001015024.2)
• Control:

  Human Spleen Whole Tissue Lysate (Adult Whole Normal)
  OSBPL5 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22241858).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OSBPL5 Antibody

• EC 1.2.1
• EC 2.7.7.6
• FLJ42929
• KIAA1534oxysterol-binding protein-related protein 5
• OBPH1
• ORP-5
• ORP5oxysterol-binding protein homologue 1
• OSBP-related protein 5
• oxysterol binding protein-like 5
• oxysterol-binding protein homolog 1
• Oxysterol-binding protein homolog 1;

Background

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OSBPL5 Antibody (NB100-57071)(3)

Publications using NB100-57071

• Kattan WE, Chen W, Ma X et al. Targeting plasma membrane phosphatidylserine content to inhibit oncogenic KRAS function Life Sci Alliance. 2019-10-01 [PMID: 31451509]
• Storey SM, McIntosh AL, Huang H et al. Intracellular cholesterol-binding proteins enhance HDL-mediated cholesterol uptake in cultured primary mouse hepatocytes Am J Physiol Gastrointest Liver Physiol 2012-04-15 [PMID: 22241858] (WB, Mouse)
• Higashimoto K, Soejima H, Yatsuki H et al. Characterization and Imprinting Status of OBPH1/Obph1 Gene: Implications for an Extended Imprinting Domain in Human and Mouse. Genomics 2002-12-01 [PMID: 12504849]

Bioinformatics

• Gene Symbol: OSBPL5
• Entrez:
  • 114879(Human)
  • 79196(Mouse)
• Uniprot:
  • NP_065947(Human)