OSBPL11 Antibody - BSA Free

Western Blot: OSBPL11 Antibody [NBP2-36545] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiORP11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: OSBPL11 Antibody [NBP2-36545] - Detection of human ORP11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP11 antibody NBP2-36545 used for IP at 6 ug per reaction. ORP11 was also immunoprecipitated by rabbit anti-ORP11 antibody NBP2-36544. For blotting immunoprecipitated ORP11, NBP2-36545 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

OSBPL11 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by the antibody maps to a region between residue 697 to 747 of human Oxysterol Binding Protein-Like 11 using the numbering given in entry NP_073613.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OSBPL11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:2000 - 1:10000
• Application Notes: Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OSBPL11 Antibody - BSA Free

• FLJ13012
• FLJ13164
• ORP11OSBP-related protein 11
• ORP-11TCCCIA00292
• OSBP12
• oxysterol binding protein-like 11
• oxysterol-binding protein-related protein 11

Background

OSBPL11 encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OSBPL11
• Uniprot:
  • NP_073613.2(Human)