Ornithine Carbamoyltransferase Antibody - BSA Free

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody [NBP1-31582] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Western Blot: Ornithine Carbamoyltransferase Antibody [NBP1-31582] - Hepatic metabolic response during infection. Mice (n=4/group) matched for intake +/- infection with PR8 influenza were euthanized at Day 5 and livers were harvested. Immunoblot of CPS1 and OTC (Ornithine Carbamoyltransferase) protein. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/cgi/doi/10.1242/dmm.013003) licensed under a CC-BY license.

Western Blot: Ornithine Carbamoyltransferase Antibody [NBP1-31582] - OTC enzyme & biochemical parameters in spf-J males.Spf-J & WT were maintained on a regular chow diet (22% protein) & euthanized at 8 weeks. Blood was collected by retro-orbital bleeding with plasma separated & stored at −80°C until use. Tissues were snap frozen & stored at −80°C until use. A) mRNA level in spf-J (N = 4) & WT (N = 4) livers. B) Immunoblot of OTC protein. C) OTC enzyme activity in liver homogenates from spf-j (N = 6) & WT (N = 4). D) Plasma ammonia in spf-j (N = 7) & WT (N = 6). E) Plasma orotate in spf-j (N = 5) & WT (N = 6). Hatched bar indicates P < 0.05. WT – wild-type. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116594), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ornithine Carbamoyltransferase antibody (NBP1-31582) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ornithine Carbamoyltransferase antibody (NBP1-31582) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ornithine Carbamoyltransferase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Ornithine Carbamoyltransferase Antibody - BSA Free (NBP1-31582) is a polyclonal antibody validated for use in IHC and WB. Anti-Ornithine Carbamoyltransferase Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Ornithine Carbamoyltransferase. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Predicted Species: Rat (93%), Porcine (94%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OTC
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:30000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Ornithine Carbamoyltransferase Antibody - BSA Free

• EC 2.1.3
• EC 2.1.3.3
• MGC129967
• MGC129968
• MGC138856
• OCTD
• Ornithine Carbamoyltransferase
• ornithine carbamoyltransferase, mitochondrial
• Ornithine Transcarbamylase
• OTC
• OTCase

Background

This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

&#9888; WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Ornithine Carbamoyltransferase Antibody (NBP1-31582)(5)

Publications using NBP1-31582

• Dryja P, Curtsinger HD, Bartee MY, Bartee E Defects in intratumoral arginine metabolism attenuate the replication and therapeutic efficacy of oncolytic myxoma virus Journal for immunotherapy of cancer 2023-06-01 [PMID: 37270180] (WB, Mouse, Primate)
• Soria L, Perocheau D, De Sabbata G et al. Beclin-1-mediated activation of autophagy improves proximal and distal urea cycle disorders EMBO Mol Med 2020-12-28 [PMID: 33369168] (Western Blot, Mouse)
• Tarasenko TN, Rosas OR, Singh LN et al. A New Mouse Model of Mild Ornithine Transcarbamylase Deficiency (spf-j) Displays Cerebral Amino Acid Perturbations at Baseline and upon Systemic Immune Activation PLoS ONE. 2015-02-04 [PMID: 25647322] (WB, Mouse)
• Yoon JC, Ling AJ, Isik M et al. GLTSCR2/PICT1 links mitochondrial stress and Myc signaling. Proc Natl Acad Sci U S A. 2014-02-20 [PMID: 24556985] (WB, Human)
• Mc Guire P, Tarasenko TN, Wang T et al. Acute metabolic decompensation due to influenza in a mouse model of ornithine transcarbamylase deficiency. Dis Model Mech. 2013-11-21 [PMID: 24271778] (WB, Mouse)

Bioinformatics

• Gene Symbol: OTC
• Entrez:
  • 5009(Human)
• Uniprot:
  • P00480()