Rat Oncostatin M/OSM ELISA Kit (Colorimetric)

ELISA: Rat Oncostatin M/OSM ELISA Kit (Colorimetric) [NBP2-68081] - Samples were spiked with high concentrations of Rat Oncostatin M/OSM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Oncostatin M/OSM ELISA Kit (Colorimetric) [NBP2-68081] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Rat Oncostatin M/OSM ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat OSM in samples. No significant cross-reactivity or interference between Rat OSM and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.63%
• Intra-Assay: CV% < 4.76%
• Spike Recovery: 86-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: OSM

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Oncostatin M/OSM ELISA Kit (Colorimetric)

• MGC20461
• oncostatin M
• oncostatin-M
• OSM

Background

Oncostatin M is a 252 amino acid protein that is 28 kDa, acts as growth regulator inhibiting the proliferation of a number of tumor cell lines and stimulating the proliferation of AIDS-KS cells; regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells; uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST), and is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. This protein is being studied for its involvement in several diseases and disorders including arterial occlusive disease, peripheral arterial occlusive disease, leukemia, kaposi's sarcoma, abdominal aortic aneurysm, status epilepticus, primary cutaneous amyloidosis, aortic aneurysm, bullous pemphigoid, neonatal jaundice, cutaneous malignant melanoma, plasmacytoma, systemic mastocytosis, mastocytosis, cervical squamous cell carcinoma, systemic lupus erythematosus, allergic rhinitis, squamous cell carcinoma, and lupus erythematosus. The protein has been linked to immune response IL-3 activation and signaling pathway, immune response oncostatin M signaling via JAK-Stat in human cells, development thrombopoetin signaling via JAK-STAT pathway, immune response Oncostatin M signaling via MAPK in human cells, MAPK signaling, endothelin-1 signaling pathway, molecular mechanisms of cancer, antioxidant action of vitamin-C, and Rho Family GTPases pathways where it interacts with IL6ST, TNNT1, LIFR, COL4A4, and COL4A6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Oncostatin M/OSM ELISA Kit (NBP2-68081)(1)

Publication using NBP2-68081

• Negrini K The Role of Oncostatin M in Exercise-Induced Delayed Latency of Breast Cancer Thesis 2023-01-01 (ELISA)

FAQs for Oncostatin M/OSM ELISA Kit (NBP2-68081). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: OSM